Department of Thoracic Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Shandong University, Jinan, China.
Department of Nursing, Shandong Medical College, Jinan, China.
Thorac Cancer. 2018 May;9(5):516-522. doi: 10.1111/1759-7714.12598. Epub 2018 Mar 25.
Signal transducer and activator of transcription 3 (STAT3) is constitutively activated in several malignancies. Here, we define the correlation between STAT3 expression and lymph node micrometastasis of early-stage non-small cell lung cancer. Then we highlight some possibilities associated with developing a way to detect tumor micrometastasis and an anticancer drug that might therapeutically inhibit the STAT3 signaling pathway.
The samples were collected from 50 patients with early-stage non-small cell lung cancer and 50 patients with benign lung tumors. Mucin 1 mRNA expression was evaluated to determine lymph node micrometastasis status. STAT3 mRNA, STAT3 protein, and phosphorylated STAT3 protein expression were evaluated through reverse transcription polymerase chain reaction, western blot, and immunohistochemistry, respectively. Measurement data was represented as mean ± standard deviation, and the t-rest or F-test were used. The χ -test was used in enumeration data. Logistic regression analysis was carried out to determine the independent risk factors influencing lymph node micrometastasis.
STAT3 mRNA and proteins expression were correlated with lymph node micrometastasis (P < 0.05). Logistic regression analysis revealed STAT3 protein overexpression and the differentiation degree of tumors were independent risk factors for lymph node micrometastasis.
Overexpression of STAT3 might promote lymphatic micrometastasis of early-stage non-small cell lung cancer and might be a clinical predictor of lymph node micrometastasis.
信号转导子和转录激活子 3（STAT3）在几种恶性肿瘤中持续激活。在这里，我们定义了 STAT3 表达与早期非小细胞肺癌淋巴结微转移之间的相关性。然后，我们强调了一些与开发检测肿瘤微转移和抗癌药物的方法相关的可能性，该药物可能在治疗上抑制 STAT3 信号通路。
从 50 例早期非小细胞肺癌患者和 50 例良性肺肿瘤患者中收集样本。通过逆转录聚合酶链反应、western blot 和免疫组织化学分别评估黏蛋白 1 mRNA 表达以确定淋巴结微转移状态。评估 STAT3 mRNA、STAT3 蛋白和磷酸化 STAT3 蛋白的表达。计量资料用均数±标准差表示，采用 t 检验或 F 检验。计数资料采用 χ 2 检验。采用 Logistic 回归分析确定影响淋巴结微转移的独立危险因素。
STAT3 mRNA 和蛋白表达与淋巴结微转移相关（P<0.05）。Logistic 回归分析显示，STAT3 蛋白过表达和肿瘤的分化程度是淋巴结微转移的独立危险因素。
STAT3 的过表达可能促进早期非小细胞肺癌的淋巴微转移，并且可能是淋巴结微转移的临床预测因子。
