Biology Department, Faculty of Science and Arts, Al Ula, Taibah University, Almadinah Almunawwarah, Saudi Arabia.
Zoology Department, Faculty of Science, Sohag University, Sohag, Egypt.
Chem Biol Drug Des. 2018 Jul;92(1):1288-1300. doi: 10.1111/cbdd.13191. Epub 2018 Apr 16.
The objective of this study was to synthesize Gibberellic lanthanide complex and evaluate its biological activity to reduce the Gibberellic acid toxicity on liver and kidney. The new bis(Gibberellic)-nitro-terbium(III) complex was characterized by different analytical methods: elemental analyses, UV-Vis, molar ratio, fluorescence, FT-IR, and TGA-DTA measurements. Thirty newborns were classified into three groups control, Gibberellic acid, and Terbium gibberellic acid complex. Livers and kidneys of studied groups proceed for general histology and immunohistochemical staining of Cyr61, cytochrome C, and TNFR2. From the absorption titration measurements, the binding constants of DNA with Tb(III)-(GA) complex and free ligand were found to be 3.9 × 10 and 2.1 × 10 m , respectively, with the stoichiometry of 1:1. Hypochromism was observed from the absorption titration experiment which indicates the intercalation of Tb(III)-(GA) complex between the base pairs of DNA. Gibberellic acid-treated group showed alteration in the histological picture of livers and kidneys that accompanied with the reduction in the expression of Cyr61, cyt C, and TNFR2. The amelioration was observed in Gibberellic acid complex with Terbium group. The study concluded that Terbium gibberellic complex is less dangerous effects than Gibberellic acid alone.
本研究的目的是合成赤霉素镧系配合物并评价其生物活性，以降低赤霉素酸对肝脏和肾脏的毒性。新的双（赤霉素）-硝基-铽（III）配合物通过不同的分析方法进行了表征：元素分析、紫外可见、摩尔比、荧光、FT-IR 和 TGA-DTA 测量。将 30 名新生儿分为三组：对照组、赤霉素组和铽赤霉素配合物组。对研究组的肝脏和肾脏进行常规组织学和 Cyr61、细胞色素 C 和 TNFR2 的免疫组织化学染色。从吸收滴定测量中，发现 Tb(III)-(GA)配合物与游离配体与 DNA 的结合常数分别为 3.9×10 和 2.1×10 m ，其化学计量比为 1:1。吸收滴定实验观察到的减色效应表明 Tb(III)-(GA)配合物插入 DNA 的碱基对之间。赤霉素酸处理组的肝脏和肾脏组织学图片发生改变，同时 Cyr61、细胞色素 C 和 TNFR2 的表达减少。在赤霉素酸与铽的配合物组中观察到改善。研究结论是，铽赤霉素配合物的危险效应比单独的赤霉素酸小。
