Liu Lulu, Xu Yi, Wang Renjie, Cui Feiyun, Liu Haitao, Chen Li
School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400030, China.
Key Laboratory for Optoelectronic Technology & System of Ministry of Education, Chongqing University, Chongqing 400044, China.
Sheng Wu Gong Cheng Xue Bao. 2018 Mar 25;34(3):320-333. doi: 10.13345/j.cjb.170264.
Biofilms are microbial communities composed of bacteria cells and self-produced extracellular polymeric substances and lead to antibiotic resistance, forming a great threat in the fields of medicine, food industries and so on. The formation of biofilms is a multi-stage dynamic process regulated by multiple mechanisms. Recently, researchers paid much more attention to the related research areas of biofilms, especially the detection methods of biofilms. This paper introduced the characteristics and formation process of biofilms as well as the effects of quorum sensing on biofilms development. Meanwhile, we discussed the detection methods of biofilms, especially electrochemical impedance spectroscopy (EIS) in detection of biofilms in detail. Furthermore, we reviewed and predicted that microfluidic chips based EIS served as an in-situ and online monitoring method for biofilms growth.
生物膜是由细菌细胞和自身产生的细胞外聚合物组成的微生物群落，会导致抗生素耐药性，在医学、食品工业等领域构成巨大威胁。生物膜的形成是一个由多种机制调控的多阶段动态过程。近年来，研究人员对生物膜的相关研究领域给予了更多关注，尤其是生物膜的检测方法。本文介绍了生物膜的特性和形成过程以及群体感应对生物膜发育的影响。同时，我们讨论了生物膜的检测方法，特别是详细阐述了电化学阻抗谱（EIS）在生物膜检测中的应用。此外，我们回顾并预测基于微流控芯片的EIS可作为生物膜生长的原位在线监测方法。
