Chen Y, Ji X Y, Fan Y Y, Yu L S
Department of Forensic Medicine, Wenzhou Medical University, Wenzhou 325035, China.
Judicial Forensic Center, Wenzhou Medical University, Wenzhou 325035, China.
Fa Yi Xue Za Zhi. 2018 Feb;34(1):7-12. doi: 10.3969/j.issn.1004-5619.2018.01.002. Epub 2018 Feb 25.
To investigate FoxO1 expression and its time-dependent changes during the skin incised wound healing.
After the establishment of the skin incised wound model in mice, the FoxO1 expression of skin in different time periods was detected by immunohistochemistry and Western blotting.
Immunohistochemistry staining showed that FoxO1 was weakly expressed in a few fibroblasts of epidermis, hair follicles, sebaceous glands, vessel endothelium and dermis in the control group. The FoxO1 expression was enhanced in the epidermis and skin appendages around the wound during 6-12 h after injury, which could be detected in the infiltrating neutrophils and a small number of monocytes. FoxO1 was mainly expressed in monocytes during 1-3 d after injury, and in neovascular endothelial cells and fibroblasts during 5-10 d. On the 14th day after injury, the FoxO1 expression still could be detected in a few fibroblasts. The Western blotting results showed that the FoxO1 expression quantity of the tissue samples in injury group was higher than in control group. The FoxO1 expression peaked at 12 h and 7 d after injury.
FoxO1 is time-dependently expressed in skin wound healing, which can be a useful marker for wound age determination.
研究FoxO1在皮肤切割伤口愈合过程中的表达及其时间依赖性变化。
建立小鼠皮肤切割伤口模型后，采用免疫组织化学和蛋白质印迹法检测不同时间段皮肤中FoxO1的表达。
免疫组织化学染色显示，对照组中FoxO1在表皮、毛囊、皮脂腺、血管内皮和真皮的少数成纤维细胞中弱表达。损伤后6 - 12小时，伤口周围表皮和皮肤附属器中FoxO1表达增强，在浸润的中性粒细胞和少量单核细胞中可检测到。损伤后1 - 3天，FoxO1主要在单核细胞中表达，5 - 10天在新生血管内皮细胞和成纤维细胞中表达。损伤后第14天，仍可在少数成纤维细胞中检测到FoxO1表达。蛋白质印迹结果显示，损伤组组织样本中FoxO1表达量高于对照组。FoxO1表达在损伤后12小时和7天达到峰值。
FoxO1在皮肤伤口愈合过程中呈时间依赖性表达，可作为判定伤口时间的有用标志物。
