Department of Oncology, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100026, China.
Department of Orthopedics, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China.
Life Sci. 2018 Jun 1;202:98-102. doi: 10.1016/j.lfs.2018.03.043. Epub 2018 Mar 22.
The present animal model of tumor neovascularization most often used by researchers is zebrafish. For studies on human breast cancer cell neovascularization, a new animal model was established to enable a more convenient study of tumor neovascularization.
A sodium alginate-gelatin blend gel system was used to design the new animal model. The model was established using rabbit corneal pouch implantation. Then, the animal model was validated by human breast cancer cell lines MCF-7-Kindlin-2 and MCF-7-CMV.
The experiment intuitively observed the relationship between tumor and neovascularization, and demonstrated the advantages of this animal model in the study of tumor neovascularization.
The use of sodium alginate-gelatin blends to establish tumor neovascularization in a rabbit corneal pouch is a novel and ideal method for the study of neovascularization. It may be a better animal model for expanding the research in this area.
研究人员目前常用的肿瘤血管生成动物模型多为斑马鱼。为了研究人类乳腺癌细胞血管生成，建立了一种新的动物模型，以更方便地研究肿瘤血管生成。
采用海藻酸钠-明胶混合凝胶系统设计新型动物模型。通过兔角膜囊袋植入建立模型。然后，使用人乳腺癌细胞系 MCF-7-Kindlin-2 和 MCF-7-CMV 对动物模型进行验证。
实验直观地观察到肿瘤与新生血管之间的关系，并证明了该动物模型在肿瘤血管生成研究中的优势。
使用海藻酸钠-明胶混合物在兔角膜囊中建立肿瘤血管生成是研究新生血管生成的一种新颖且理想的方法。它可能是扩展该领域研究的更好的动物模型。
