大豆十肽通过多巴胺受体诱导果蝇后腿梳理行为。

Soy undecapeptide induces Drosophila hind leg grooming via dopamine receptor.

机构信息

Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan; Department of Biotechnology and Genetic Engineering, Jahangirnagar University, Savar, Dhaka 1342, Bangladesh.

Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Japan.

出版信息

Biochem Biophys Res Commun. 2018 May 15;499(3):454-458. doi: 10.1016/j.bbrc.2018.03.162. Epub 2018 Mar 27.

DOI:10.1016/j.bbrc.2018.03.162

PMID:29577906

Abstract

β-Conglycinin α subunit (323-333) [βCGα(323-333)] is an exogenous neuromodulating undecapeptide found from enzymatic digest of β-conglycinin, a soy major storage protein by mice behavior tests. We investigated effect of βCGα(323-333) on Drosophila behavior. Oral administration of βCGα(323-333) in Drosophila increased hind leg grooming, which may act through specific sets of neurons. It was reported that dopamine receptor (DopR) meditates hind leg grooming, and we tested involvement of DopR in βCGα(323-333)-induced hind leg grooming by using DopR knockout flies. In the wild type but not in the DopR-knockout flies, βCGα(323-333) increased hind leg grooming. These results suggest that βCGα(323-333) induces hind leg grooming via activating the DopR. This is the first report showing that exogenously administered peptide changes fly behaviors.

摘要

β-伴大豆球蛋白 α 亚基（323-333）[βCGα(323-333)]是一种外源性神经调节十肽，通过对大豆主要储存蛋白β-伴大豆球蛋白的酶解，通过小鼠行为测试发现。我们研究了βCGα(323-333)对果蝇行为的影响。口服给予果蝇βCGα(323-333)可增加后腿梳理行为，这可能是通过特定的神经元群起作用的。据报道，多巴胺受体（DopR）介导后腿梳理，我们通过使用 DopR 敲除果蝇来测试 DopR 在βCGα(323-333)诱导后腿梳理中的作用。在野生型果蝇中，但在 DopR 敲除果蝇中，βCGα(323-333)增加了后腿梳理行为。这些结果表明，βCGα(323-333)通过激活 DopR 诱导后腿梳理。这是第一个表明外源性给予的肽可以改变果蝇行为的报告。