Maéno M, Nakamura T, Tochinai S, Katagiri C
Transplantation. 1987 Aug;44(2):308-14. doi: 10.1097/00007890-198708000-00025.
The triploid J strain Xenopus laevis (MHC haplotype, j/j/j) were thymectomized as early larvae, and in the adult stage each animal was given a pair of thymuses or lymphocytes from semiallogeneic diploid donor frogs (j/k). The alloreactivity of host frogs was restored to third-party donors in terms of skin graft rejection and mixed leukocyte reaction, but there was specific unresponsiveness against the k haplotype. Grafting of heavily irradiated (10,000 rads) thymuses also restored host reactivity with induction of tolerance to the k haplotype. In the latter frogs, all thymic and splenic lymphocytes were of host origin. Injection of splenocytes from the restored frogs into secondary thymectomized J frogs not only restored immunocompetence but also transferred specific tolerance to the k haplotype. Injection of lymphocytes from the restored frogs to normal frogs failed to transfer specific tolerance by both in vivo and in vitro immune tests. The results suggest that a selective deletion of the T cell population reactive to the k haplotype was maintained in the T-cell-restored frogs.
三倍体J品系非洲爪蟾（MHC单倍型，j/j/j）在幼体早期进行胸腺切除，成年后，每只动物接受一对来自半同种异体二倍体供体蛙（j/k）的胸腺或淋巴细胞。就皮肤移植排斥和混合淋巴细胞反应而言，宿主蛙的同种异体反应性恢复到对第三方供体的反应，但对k单倍型存在特异性无反应性。移植经过重度照射（10000拉德）的胸腺也能恢复宿主反应性，并诱导对k单倍型的耐受性。在后者的蛙中，所有胸腺和脾脏淋巴细胞均来自宿主。将恢复后的蛙的脾细胞注射到再次进行胸腺切除的J蛙中，不仅恢复了免疫能力，还转移了对k单倍型的特异性耐受性。通过体内和体外免疫试验，将恢复后的蛙的淋巴细胞注射到正常蛙中均未能转移特异性耐受性。结果表明，在T细胞恢复的蛙中，对k单倍型有反应的T细胞群体被选择性清除。
