Nikpour Fatemeh, Tayefi Hamid, Mohammadnejad Daryosh, Akbarzadeh Abolfazl
Department of Histological Sciences, Tabriz University of Medical Sciences, Tabriz, Iran. Email:
Asian Pac J Cancer Prev. 2018 Mar 27;19(3):683-687. doi: 10.22034/APJCP.2018.19.3.683.
The present study was designed to determine changes in spermatogenesis in adult mice after an intraperitoneal injection of vincristine. The effect of a GnRH antagonist synchronous to chemotherapy, which might protect spermatogenesis by halting cell division in spermatogenic cells, was also investigated. Method and Materials: A total of 30 adult male mice were studied in three equal groups of ten. In the V group, a single dose of the chemotherapy drug vincristine was injected intraperitonally at 1.5 mg/kg. In the V+C, group, the injection of Cetrorelix was started, and one week before to one week after vincristine injection continued ( for 3 weeks). Controls received no treatment. Samples were taken from the testicles, and fixed in Boueins fixative for light microscopy. Results: Comparing the mean number of Sertoli and spermatogony cells and the rate of spermatogenesis index (SI) in the V group with controls showed significant differences,which were not evident in the V+C group. Conclusion: According to the results, the cetrorelix antagonist (GnRH) could largely prevent side effects of vincristine administration regarding seminiferous tubules.
本研究旨在确定成年小鼠腹腔注射长春新碱后精子发生的变化。还研究了与化疗同步使用的GnRH拮抗剂的作用，其可能通过阻止生精细胞的细胞分裂来保护精子发生。方法和材料：总共30只成年雄性小鼠被分为三组，每组十只。在V组中，以1.5mg/kg的剂量腹腔注射单剂量化疗药物长春新碱。在V+C组中，开始注射西曲瑞克，并在长春新碱注射前一周至注射后一周持续注射（共3周）。对照组不接受治疗。从睾丸取样，用Bouin固定液固定用于光学显微镜检查。结果：将V组的支持细胞和精原细胞的平均数量以及精子发生指数（SI）与对照组进行比较，显示出显著差异，而在V+C组中不明显。结论：根据结果，西曲瑞克拮抗剂（GnRH）在很大程度上可以预防长春新碱给药对生精小管的副作用。
