School of Ophthalmology & Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou, 325027, China; Wenzhou Institute of Biomaterials and Engineering, Chinese Academy of Sciences, Wenzhou, 32500, China.
School of Ophthalmology & Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou, 325027, China.
Carbohydr Polym. 2018 Jun 1;189:65-71. doi: 10.1016/j.carbpol.2018.02.022. Epub 2018 Feb 9.
The over- and inefficient release of antibiotics from common delivery systems causes the development of drug-resistant bacteria. In the present work, methoxy poly(ethylene glycol)-poly(ε-caprolactone)-chitosan/montmorillonite (MPC/MMT) hybrid multilayer films were constructed for bacterial infections and pH-dependent release of the hydrophobic drug triclosan (TCA). The thickness of the (MPC-T/MMT) multilayer films was 384.4 ± 26.5 nm, and the TCA loading dosage was 2.4 μg/cm. Staphylococcus aureus, Escherichia coli and Staphylococcus epidermidis were used in the antibacterial tests. pH responsive TCA release from the prepared multilayer films was examined by measuring the bactericidal activity of the films after immersion in PBS (pH 7.4) or MES (pH 5.5) and zone of inhibition on nutrient agar. In vitro bacterial shake-flask, zone of inhibition and live/dead staining results demonstrated the high sterilization efficiency of the films. Furthermore, cell biocompatibility measurements toward L929 fibroblasts and human lens epithelial cells showed no adverse effects of the multilayer film.
从常见的输送系统过度和低效地释放抗生素会导致耐药细菌的产生。在本工作中，构建了甲氧基聚乙二醇-聚（ε-己内酯）-壳聚糖/蒙脱土（MPC/MMT）杂化多层膜，用于细菌感染和疏水性药物三氯生（TCA）的 pH 依赖性释放。（MPC-T/MMT）多层膜的厚度为 384.4±26.5nm，TCA 的载药量为 2.4μg/cm。使用金黄色葡萄球菌、大肠杆菌和表皮葡萄球菌进行了抗菌测试。通过测量在 PBS（pH 7.4）或 MES（pH 5.5）中浸泡后膜的杀菌活性以及在营养琼脂上的抑菌圈，研究了制备的多层膜对 pH 响应的 TCA 释放。体外细菌摇瓶、抑菌圈和死活染色结果表明了膜的高杀菌效率。此外，对 L929 成纤维细胞和人晶状体上皮细胞的细胞生物相容性测量表明，多层膜没有不良影响。
