用于研究IGFBP6基因在乳腺癌转移中作用的体外模型
In Vitro Model for Studying of the Role of IGFBP6 Gene in Breast Cancer Metastasizing.
作者信息
Nikulin S V, Raigorodskaya M P, Poloznikov A A, Zakharova G S, Schumacher U, Wicklein D, Stürken C, Riecken K, Fomicheva K A, Alekseev B Ya, Shkurnikov M Yu
机构信息
BioClinicum Center, Moscow, Russia.
Moscow Institute of Physics and Technology (MIPT), Moscow, Russia.
出版信息
Bull Exp Biol Med. 2018 Mar;164(5):688-692. doi: 10.1007/s10517-018-4060-7. Epub 2018 Mar 27.
IGFBP6 gene plays an important role in the pathogenesis of breast cancer. In this work, we performed knockdown of IGFBP6 gene in MDA-MB-231 cells and obtained a stable cell line. Knockdown of IGFBP6 gene was confirmed by the real-time PCR. The influence of IGFBP6 gene on migration and proliferation of breast cancer cells was studied. Knockdown of IGFBP6 gene reduced migration activity of MDA-MB-231 cells and increased their proliferation rate. This in vitro cell model can be used for the further analysis of the role of IGFBP6 gene in the pathogenesis of breast cancer.
胰岛素样生长因子结合蛋白6（IGFBP6）基因在乳腺癌发病机制中起重要作用。在本研究中，我们在MDA-MB-231细胞中敲低了IGFBP6基因，并获得了稳定的细胞系。通过实时PCR证实了IGFBP6基因的敲低。研究了IGFBP6基因对乳腺癌细胞迁移和增殖的影响。敲低IGFBP6基因降低了MDA-MB-231细胞的迁移活性并提高了其增殖率。这种体外细胞模型可用于进一步分析IGFBP6基因在乳腺癌发病机制中的作用。
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