Koistinen Jaana, Sjöblom Mervi, Spilling Kristian
Tvärminne Zoological Station, University of Helsinki, Hanko, Finland.
Finnish Environment Institute, Helsinki, Finland.
Methods Mol Biol. 2020;1980:71-80. doi: 10.1007/7651_2018_128.
Nitrogen (N) is one of the key nutrients for algal growth and is an integral part of many cellular components, for example in proteins. Being able to determine the inorganic and organic pools of N is consequently critical for algal cultivation. In this chapter we present the methods we use for determining dissolved inorganic nitrogen (DIN), dissolved organic nitrogen (DON), and particulate organic nitrogen (PON). The methods presented here for DIN rely on colorimetric methods and those of DON and PON on filtration and high temperature catalytic oxidation.
氮（N）是藻类生长的关键营养素之一，是许多细胞成分（如蛋白质）的组成部分。因此，能够测定氮的无机和有机库对于藻类培养至关重要。在本章中，我们介绍了用于测定溶解无机氮（DIN）、溶解有机氮（DON）和颗粒有机氮（PON）的方法。这里介绍的DIN测定方法依赖于比色法，而DON和PON的测定方法则依赖于过滤和高温催化氧化。
