Binninger E A, Schachtschabel D O, Rohen J W
Institut für Physiologische Chemie, Philipps-Universität, Marburg, Federal Republic of Germany.
Exp Eye Res. 1987 Jul;45(1):169-77. doi: 10.1016/s0014-4835(87)80087-8.
Addition of hyaluronic acid (50-200 micrograms ml-1) to the defined, serum-free media of cultured human trabecular-meshwork cells resulted in an increase of glycosaminoglycan (GAG) synthesis as measured by the incorporation of [14C]glucosamine. Lesser stimulatory effects were exerted by dermatan sulfate and chondroitin-4- or -6-sulfate. Nearly 90% of the labeled GAGs were found to be exerted into the medium and ea. 10% were associated with the cell layer. Mainly hyaluronic acid synthesis was stimulated by the exogenous GAGs. Analysis of the GAG-pattern revealed that exogenous hyaluronic acid stimulated hyaluronic acid synthesis (positive feedback), while exogenous dermatan sulfate and chondroitin sulfate had additional effects on chondroitin sulfate synthesis. Cell growth of these cultures, which exhibited a limited proliferative capacity (ca. 18 population doublings during their life span) was not affected by the GAG treatment. Thus, exogenous hyaluronic acid and to a lower degree dermatan sulfate or chondroitin sulfate appeared to interfere with the GAG-metabolism of these human trabecular-meshwork cells in culture.
在培养人小梁网细胞的特定无血清培养基中添加透明质酸（50 - 200微克/毫升），通过[14C]葡萄糖胺掺入法测定，可导致糖胺聚糖（GAG）合成增加。硫酸皮肤素和硫酸软骨素 - 4或 - 6对其刺激作用较小。发现近90%的标记GAG分泌到培养基中，约10%与细胞层相关。外源性GAG主要刺激透明质酸合成。对GAG模式的分析表明，外源性透明质酸刺激透明质酸合成（正反馈），而外源性硫酸皮肤素和硫酸软骨素对硫酸软骨素合成有额外影响。这些培养物的细胞生长具有有限的增殖能力（在其生命周期内约18次群体倍增），不受GAG处理的影响。因此，外源性透明质酸以及较低程度的硫酸皮肤素或硫酸软骨素似乎会干扰培养中这些人小梁网细胞的GAG代谢。
