用色谱聚焦法对变形链球菌OMZ176葡糖基转移酶的纯化及特性分析

Purification and characterization of glucosyltransferase from Streptococcus mutans OMZ176 with chromatofocusing.

作者信息

Kametaka S, Hayashi S, Miyake Y, Suginaka H

机构信息

Research and Development Division, Rohto Pharmaceutical Co. Ltd., Osaka, Japan.

出版信息

Microbios. 1987;51(206):29-35.

PMID:2958682

Abstract

A crude glucosyltransferase (GTase) preparation of Streptococcus mutans OMZ176 was fractionated by chromatography on a chromatofocusing column. It was separated into three major fractions. Fractions 1 and 3 mainly synthesized water-soluble glucan (SG) without primer dextran T-10. Dextransucrase activity of fraction 1 was not increased by the primer, although that of fraction 3 was increased. Fraction 2 synthesized only water-insoluble glucan (IG) in the absence of a primer, but mutansucrase activity of this fraction was greatly increased dose-dependently by the addition of a primer. The SG and IG synthesized by fraction 1 were rich in alpha-1,6 glucosidic linkages. On the other hand, about 80% of glucose residues of the IG synthesized by fraction 2 were alpha-1,3 linked. Both SG and IG synthesized by fraction 3 contained highly branched structures.

摘要

变形链球菌OMZ176的粗制葡糖基转移酶（GTase）制剂通过在色谱聚焦柱上进行色谱分离。它被分离成三个主要部分。部分1和部分3主要合成无引物葡聚糖T-10的水溶性葡聚糖（SG）。部分1的葡糖基转移酶活性不会因引物而增加，尽管部分3的活性会增加。部分2在没有引物的情况下仅合成水不溶性葡聚糖（IG），但该部分的变聚糖酶活性通过添加引物而呈剂量依赖性地大幅增加。部分1合成的SG和IG富含α-1,6糖苷键。另一方面，部分2合成的IG中约80%的葡萄糖残基是α-1,3连接的。部分3合成的SG和IG都含有高度分支的结构。