Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai 400076, India.
Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai 400076, India.
Biosens Bioelectron. 2018 Jul 1;110:38-43. doi: 10.1016/j.bios.2018.03.040. Epub 2018 Mar 20.
An optimum copper concentration in environment is highly desired for all forms of life. We have developed an ultrasensitive copper sensor which functions from femto to micro molar concentration accurately (R = 0.98). The sensor is based on denatured antibody immunoglobulin G (IgG), immobilized on polyaniline (PAni) which in turn is the coating on the core of an optical fiber. The sensing relies on changes in evanescent wave absorbance in the presence of the analyte. The sensor showed excellent selectivity towards Cu (II) ions over all other metal ions. The sensor was tested with lake and marine water samples to determine unknown concentrations of copper ions and the recovery results were within 90-115%, indicating reasonable accuracy. We further integrated the fiber-optic sensor with a miniaturized hand-held instrumentation platform to develop an accurate and field deployable device which can broadly be applicable to determine Cu (II) concentration in a wide range of systems - natural water bodies, soil as well as blood serum.
对于所有形式的生命来说，环境中的最佳铜浓度是非常理想的。我们开发了一种超灵敏的铜传感器，能够准确地测量从皮摩尔到微摩尔浓度范围内的铜（R = 0.98）。该传感器基于变性的抗体免疫球蛋白 G（IgG），固定在聚苯胺（PAni）上，而聚苯胺又涂覆在光纤的核心上。该传感器通过检测分析物存在时的消逝波吸收变化来进行感应。该传感器对铜（II）离子具有出色的选择性，优于所有其他金属离子。我们使用湖水和海水样本对传感器进行了测试，以确定未知浓度的铜离子，回收率在 90-115%之间，表明具有合理的准确性。我们进一步将光纤传感器与小型手持式仪器平台集成，开发出一种准确且可现场部署的设备，该设备可广泛应用于确定各种系统（天然水体、土壤和血清）中的 Cu（II）浓度。
