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与紫薯块茎中花青素积累相关的 microRNA 及其靶基因的鉴定()。 （注：括号部分原文缺失内容）

Identification of microRNAs and their target genes related to the accumulation of anthocyanin in purple potato tubers ().

作者信息

Wu Xiaojuan, Ma Yanhong, Wu Juan, Wang Peijie, Zhang Zhicheng, Xie Rui, Liu Jie, Fan Bobo, Wei Wei, Nie Li Zhen, Liu Xuting

机构信息

Agricultural College Inner Mongolia Agricultural University Hohhot China.

Wulanchabu Academy of Agricultural and Forest Sciences Wulanchabu China.

出版信息

Plant Direct. 2022 Jul 17;6(7):e418. doi: 10.1002/pld3.418. eCollection 2022 Jul.

DOI:10.1002/pld3.418
PMID:35865074
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9289217/
Abstract

MicroRNAs (miRNAs) are types of endogenous non-coding small RNAs found in eukaryotes that are 18-25 nucleotides long. miRNAs are considered to be key regulatory factors of the expression of target mRNA. The roles of miRNAs involved in the regulation of anthocyanin accumulation in pigmented potatoes have not been systematically reported. In this study, the differentially expressed miRNAs and their target genes involved in the accumulation of anthocyanin during different developmental stages in purple potato ( L.) were identified using small RNA (sRNA) and degradome sequencing. A total of 275 differentially expressed miRNAs were identified in the sRNA libraries. A total of 69,387,200 raw reads were obtained from three degradome libraries. The anthocyanin responsive miRNA-mRNA modules were analyzed, and 37 miRNAs and 23 target genes were obtained. Different miRNAs regulate the key enzymes of anthocyanin synthesis in purple potato. The structural genes included phenylalanine ammonia lyase, chalcone isomerase, flavanone 3-hydroxylase, and anthocyanidin 3--glucosyltransferase. The regulatory genes included WD40, MYB, and SPL9. stu-miR172e-5p_L-1R-1, stu-miR828a, stu-miR29b-4-5p, stu-miR8019-5p_L-4R-3, stu-miR396b-5p, stu-miR5303f_L-7R + 2, stu-miR7997a_L-3, stu-miR7997b_L-3, stu-miR7997c_L + 3R-5_2ss2TA3AG, stu-miR156f-5p_L + 1, stu-miR156a, stu-miR156a_R-1, stu-miR156e, stu-miR858, stu-miR5021, stu-miR828 and their target genes were validated by qRT-PCR. They play important roles in the coloration and accumulation of purple potatoes. These results provide new insights into the biosynthesis of anthocyanins in pigmented potatoes.

摘要

微小RNA（miRNA）是在真核生物中发现的一类内源性非编码小RNA，长度为18 - 25个核苷酸。miRNA被认为是靶标mRNA表达的关键调控因子。参与调控有色马铃薯中花青素积累的miRNA的作用尚未得到系统报道。在本研究中，利用小RNA（sRNA）和降解组测序鉴定了紫色马铃薯（L.）不同发育阶段参与花青素积累的差异表达miRNA及其靶基因。在sRNA文库中总共鉴定出275个差异表达的miRNA。从三个降解组文库中总共获得了69387200条原始读数。分析了花青素响应性miRNA - mRNA模块，获得了37个miRNA和23个靶基因。不同的miRNA调控紫色马铃薯中花青素合成的关键酶。结构基因包括苯丙氨酸解氨酶、查尔酮异构酶、黄烷酮3 - 羟化酶和花青素3 - 葡萄糖基转移酶。调控基因包括WD40、MYB和SPL9。通过qRT - PCR验证了stu - miR172e - 5p_L - 1R - 1、stu - miR828a、stu - miR29b - 4 - 5p、stu - miR8019 - 5p_L - 4R - 3、stu - miR396b - 5p、stu - miR5303f_L - 7R + 2、stu - miR7997a_L - 3、stu - miR7997b_L - 3、stu - miR7997c_L + 3R - 5_2ss2TA3AG、stu - miR156f - 5p_L + 1、stu - miR156a、stu - miR156a_R - 1、stu - miR156e、stu - miR858、stu - miR5021、stu - miR828及其靶基因。它们在紫色马铃薯的着色和积累中起重要作用。这些结果为有色马铃薯中花青素的生物合成提供了新的见解。

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