Zhejiang Key Lab of Crop Germplasm, Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.
Zhejiang Key Lab of Crop Germplasm, Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.
Biochem Biophys Res Commun. 2018 May 15;499(3):513-518. doi: 10.1016/j.bbrc.2018.03.179. Epub 2018 Mar 31.
PHYTOCHROME INTERACING FACTOR 3 LIKE 5 (PIL5), also named PHYTOCHROME INTERACTING FACTOR 1 (PIF1) is an important b-HLH transcription factor in Arabidopsis thaliana. Here we show that mutant of pil5-1 displays early flowering phenotype. We demonstrate that the expressions of the major flowering promoter genes [FLOWERING LOCUS T (FT), SUPPRESOR OF OVEREXPRESSION OF CO 1 (SOC1), and LEAFY (LFY)] are upregulated in the mutant of pil5-1. There is a significant increase of the mRNA of PIL5 in the mutants of co2-1, ft-10, soc1-2, and lfy-4. These changes provide the molecular evidence that PIL5 interacts with the flowering regulators to control flowering time. Moreover, it is shown in our results that PIL5 mutation mediates the increased contents of gibberellic acid (GA). Which is further supported by the qRT-PCR analysis, an increased transcriptome level of the GA biosynthesis genes (GA3ox1, GA3ox2, GA20ox1, GA20ox2, and GA20ox3) has been observed in the pil5-1 mutants as compared to the wild type. Collectively, PIL5 is involved in floral transition interacting with flowering integrators and GA.
光形态建成素互作因子 3 样 5（PIL5），也被称为光形态建成素互作因子 1（PIF1），是拟南芥中重要的 b-HLH 转录因子。在这里，我们发现 pil5-1 突变体表现出早花表型。我们证明，主要开花启动子基因[开花时间（FT）、过量表达 CO1 的抑制子（SOC1）和叶（LFY）]的表达在 pil5-1 突变体中上调。在 co2-1、ft-10、soc1-2 和 lfy-4 的突变体中，PIL5 的 mRNA 显著增加。这些变化为 PIL5 与开花调节剂相互作用以控制开花时间提供了分子证据。此外，我们的结果表明 PIL5 突变介导赤霉素（GA）含量的增加。这进一步得到了 qRT-PCR 分析的支持，与野生型相比，pil5-1 突变体中 GA 生物合成基因（GA3ox1、GA3ox2、GA20ox1、GA20ox2 和 GA20ox3）的转录组水平显著增加。总之，PIL5 参与了与开花整合因子和 GA 相互作用的花发育转变。
