College of Animal Science and Technology, Shihezi University, Shihezi 832003, China.
College of Animal Science and Technology, Shihezi University, Shihezi 832003, China.
Biochem Biophys Res Commun. 2018 May 5;499(2):321-327. doi: 10.1016/j.bbrc.2018.03.155. Epub 2018 Mar 26.
Given the important role of nutritional status for reproductive performance, we aimed to explore the potential microRNA (miRNA)-mRNA pairs and their regulatory roles associated with nutritional status in seasonal reproducing sheep. Individual ewes were treated with and without 0.3 kg/day concentrates, and the body condition score, estrus rate, and related miRNAs and target genes were compared. A total of 261 differentially expressed miRNAs were identified, including 148 hypothalamus-expressed miRNAs and 113 ovary-expressed miRNAs, and 349 target genes were predicted to be associated with nutritional status and seasonal reproduction in sheep. Ultimately, the miR-200b-GNAQ pair was screened and validated as differentially expressed, and a dual luciferase reporter assay showed that miR-200b could bind to the 3'-untranslated region of GNAQ to mediate the hypothalamic-pituitary-ovarian axis. Thus, miR-200b and its target gene GNAQ likely represent an important negative feedback loop, providing a link between nutritional status and seasonal reproduction in sheep toward enhancing reproductive performance and productivity.
鉴于营养状况对繁殖性能的重要作用，本研究旨在探讨与季节性繁殖绵羊营养状况相关的潜在微小 RNA（miRNA）-mRNA 对及其调控作用。单独给母羊投喂或不投喂 0.3kg/天的浓缩饲料，并比较体况评分、发情率以及相关的 miRNA 和靶基因。共鉴定出 261 个差异表达的 miRNA，包括 148 个下丘脑表达的 miRNA 和 113 个卵巢表达的 miRNA，预测 349 个靶基因与绵羊的营养状况和季节性繁殖有关。最终筛选并验证了 miR-200b-GNAQ 对的差异表达，双荧光素酶报告基因实验表明，miR-200b 可以与 GNAQ 的 3'-非翻译区结合，介导下丘脑-垂体-卵巢轴。因此，miR-200b 及其靶基因 GNAQ 可能代表一个重要的负反馈回路，为绵羊的营养状况与季节性繁殖之间提供了联系，以提高繁殖性能和生产力。
