Saraiva E M, Andrade A F, de Souza W
Laboratório de Ultraestrutura Celular, Instituto de Biofísica Carlos Chagas Filho, Rio de Janeiro.
Parasitol Res. 1987;73(5):411-6. doi: 10.1007/BF00538197.
Significant differences were found in the ability of resident mouse peritoneal macrophages to ingest amastigote and promastigote forms of Leishmania mexicana amazonensis. Differences in the association index of the parasites to the macrophages were also found between infective and non-infective promastigotes. Evidence was obtained suggesting that the macrophage receptor, which recognizes mannose-6-phosphate-containing units found in lysosomal enzymes, is involved in the association with the macrophage of promastigotes, but not of amastigotes. Addition of mannose-6-phosphate, its structural analogue fructose-1-phosphate, Hansenula holstii phosphomannan or the mannose-6-phosphate-containing lysosomal enzyme alpha-D-mannosidase to the interaction medium, markedly inhibits the association of the parasites with macrophages.
研究发现，驻留小鼠腹腔巨噬细胞摄取墨西哥利什曼原虫亚马逊亚种无鞭毛体和前鞭毛体的能力存在显著差异。在感染性和非感染性前鞭毛体与巨噬细胞的结合指数上也发现了差异。有证据表明，识别溶酶体酶中含甘露糖-6-磷酸单元的巨噬细胞受体参与前鞭毛体与巨噬细胞的结合，但不参与无鞭毛体与巨噬细胞的结合。向相互作用介质中添加甘露糖-6-磷酸、其结构类似物果糖-1-磷酸、霍氏假丝酵母磷酸甘露聚糖或含甘露糖-6-磷酸的溶酶体酶α-D-甘露糖苷酶，可显著抑制寄生虫与巨噬细胞的结合。
