Miljković Milica, Stefanović Aleksandra, Bogavac-Stanojević Nataša, Simić-Ogrizović Sanja, Dumić Jerka, Černe Darko, Jelić-Ivanović Zorana, Kotur-Stevuljević Jelena
Department of Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia.
Department of Nephrology, Clinical Center of Serbia, Belgrade, Serbia.
Acta Clin Croat. 2017 Dec;56(4):673-680. doi: 10.20471/acc.2017.56.04.14.
Inflammation, apoptosis and extracellular remodeling play significant roles in cardiovascular disease (CVD) underlying the major causes of mortality in renal patients. In 19 pre-dialysis patients, 21 dialysis patients and 20 control subjects, the concentrations of pentraxin-3, galectin-3, MMP-9 and TIMP-1 were determined by ELISA. CVD risk was calculated according to the Framingham risk score algorithm. Pentraxin-3 was increased in renal patients compared to healthy controls (p<0.001). In contrast, galectin-3 was reduced in hemodialysis patients compared to pre-dialysis patients and controls (p<0.001). In addition, MMP-9 and TIMP-1 were elevated in renal patients compared to controls (p<0.01 and p<0.001, respectively). Logistic regression analyses disclosed associations of galectin-3, MMP-9, pentraxin-3 and glomerular filtration with calculated CVD risk score. Combined testing of pentraxin-3, galectin-3, MMP-9 and glomerular filtration rate can discriminate renal patients with high and low risk of a coronary event.
炎症、细胞凋亡和细胞外重塑在心血管疾病（CVD）中起着重要作用，而心血管疾病是肾病患者主要的死亡原因。对19名透析前患者、21名透析患者和20名对照受试者，采用酶联免疫吸附测定法（ELISA）测定了五聚体蛋白-3、半乳糖凝集素-3、基质金属蛋白酶-9（MMP-9）和金属蛋白酶组织抑制因子-1（TIMP-1）的浓度。根据弗明汉风险评分算法计算心血管疾病风险。与健康对照相比，肾病患者的五聚体蛋白-3升高（p<0.001）。相比之下，与透析前患者和对照相比，血液透析患者的半乳糖凝集素-3降低（p<0.001）。此外，与对照相比，肾病患者的MMP-9和TIMP-1升高（分别为p<0.01和p<0.001）。逻辑回归分析揭示了半乳糖凝集素-3、MMP-9、五聚体蛋白-3和肾小球滤过与计算出的心血管疾病风险评分之间的关联。联合检测五聚体蛋白-3、半乳糖凝集素-3、MMP-9和肾小球滤过率可以区分冠心病事件高风险和低风险的肾病患者。
