Tally M, Enberg G, Li C H, Hall K
Department of Endocrinology, Karolinska Institutet, Stockholm, Sweden.
Biochem Biophys Res Commun. 1987 Sep 30;147(3):1206-12. doi: 10.1016/s0006-291x(87)80198-5.
The specificity of the type 2 insulinlike growth factor (IGF) receptor is evaluated in human placenta membranes and the human cell line K562. K562 cells have type 2 but not type 1 IGF receptors. Native IGF-2 isolated from human plasma and synthetic IGF-2 were equipotent in competing with labeled IGF-2 in both systems. Pure IGF-1 isolated from plasma, synthetic IGF-1 and recombinant IGF-1 could not crossreact with the type 2 IGF receptor in concentrations up to 1 microgram/ml in both systems. Studies on placenta membrane were done in the presence of 300 ug/ml insulin to block the type 1 IGF receptors. It is concluded that IGF-1, as well as insulin, cannot crossreact with the human type 2 IGF receptor.
在人胎盘膜和人细胞系K562中评估了2型胰岛素样生长因子（IGF）受体的特异性。K562细胞具有2型IGF受体，但没有1型IGF受体。从人血浆中分离出的天然IGF-2和合成的IGF-2在两个系统中与标记的IGF-2竞争时效力相当。从血浆中分离出的纯IGF-1、合成的IGF-1和重组IGF-1在两个系统中浓度高达1微克/毫升时都不能与2型IGF受体发生交叉反应。在存在300微克/毫升胰岛素的情况下对胎盘膜进行研究，以阻断1型IGF受体。得出的结论是，IGF-1以及胰岛素都不能与人2型IGF受体发生交叉反应。
