Department of Energy and Environment Science, Nagaoka University of Technology, 1603-1, Kamitomioka Nagaoka, Niigata, 940-2188, Japan.
Department of Chemical and Biological Engineering, National Institute of Technology, Ube College, Tokiwadai, Ube, Yamaguchi, 755-8555, Japan.
Appl Microbiol Biotechnol. 2018 May;102(10):4381-4392. doi: 10.1007/s00253-018-8864-1. Epub 2018 Mar 28.
The characteristics of the microbial community in a practical-scale down-flow hanging sponge (DHS) reactor, high in organic matter and sulfate ion concentration, and the seasonal variation of the microbial community composition were investigated. Microorganisms related to sulfur oxidation and reduction (2-27%), as well as Leucobacter (7.50%), were abundant in the reactor. Anaerobic bacteria (27-38% in the first layer) were also in abundance and were found to contribute to the removal of organic matter from the sewage in the reactor. By comparing the Simpson index, the abundance-based coverage estimator (ACE) index, and the species composition of the microbial community across seasons (summer/dry, summer/rainy, autumn/dry, and winter/dry), the microbial community was found to change in composition only during the winter season. In addition to the estimation of seasonal variation, the difference in the microbial community composition along the axes of the DHS reactor was investigated for the first time. Although the abundance of each bacterial species differed along both axes of the reactor, the change of the community composition in the reactor was found to be greater along the vertical axis than the horizontal axis of the DHS reactor.
本研究考察了实际规模下流式悬挂海绵（DHS）反应器中微生物群落的特性，该反应器中有机物和硫酸根离子浓度较高，以及微生物群落组成的季节性变化。与硫氧化和还原（2-27%）以及 Leucobacter（7.50%）相关的微生物在反应器中丰富。厌氧细菌（第一层的 27-38%）也很丰富，有助于去除反应器中污水中的有机物。通过比较 Simpson 指数、基于丰度的覆盖估计（ACE）指数和微生物群落的季节组成（夏季/干燥、夏季/雨季、秋季/干燥和冬季/干燥），发现微生物群落仅在冬季发生组成变化。除了估计季节性变化外，本研究还首次研究了 DHS 反应器沿轴微生物群落组成的差异。尽管反应器中每个细菌物种的丰度沿两个轴都有所不同，但发现反应器中群落组成的变化沿 DHS 反应器的垂直轴比水平轴更大。
