Department of Chemistry, American University of Beirut, PO Box 11-0236, Riad El Solh, Beirut, 1107 2020, Lebanon.
Mikrochim Acta. 2018 Feb 19;185(3):185. doi: 10.1007/s00604-018-2725-6.
The authors describe the preparation of gold nanoparticles (AuNPs) coated with poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol) (Pluronic F-108) by reducing Au to Au using curcumin, a natural and non-toxic food spice, in water of pH ~7 in the presence of F-108 and Ag ion. The coated AuNPs display strong resonance Rayleigh scattering (RRS) and fluorescence that results from the functionalization of the gold surface with curcumin and Pluronic F-108. The molar mass of Pluronic F-108 affects the particle size of the AuNPs formed, and small AuNPs are formed when using low molar weight F-108 that was purified by centrifugation or dialysis. The coated AuNPs were employed in an optical method for the determination of uric acid. The combination of uric acid with the AuNPs boosts both the RRS signal and the fluorescence of the AuNPs. However, higher concentrations of uric acid shift the fluorescence peak to shorter wavelengths. The method is simple, and fluorescence, best measured at excitation/emission wavelengths of 425/534 nm, increases linearly in the 50 μM to 50 mM uric acid concentration range, with a 0.14 μM detection limit which is lower than reported for other methods in the literature. Graphical abstract Pluronic F-108 capped gold nanoparticles prepared by reducing Au to Au using curcumin can estimate uric acid in 50 μM to 50 mM concentration range.
作者描述了通过使用姜黄素将金还原为金，在 pH~7 的水中制备包覆有多聚乙二醇-嵌段-聚丙二醇-嵌段-多聚乙二醇（Pluronic F-108）的金纳米粒子（AuNPs）。姜黄素和 Pluronic F-108 对金表面的功能化使得包覆的 AuNPs 具有强共振瑞利散射（RRS）和荧光。Pluronic F-108 的摩尔质量影响形成的 AuNPs 的粒径，并且当使用通过离心或透析纯化的低摩尔质量 F-108 时形成小的 AuNPs。包覆的 AuNPs 被用于尿酸的光学测定方法。尿酸与 AuNPs 的结合增强了 RRS 信号和 AuNPs 的荧光。然而，尿酸的浓度较高会将荧光峰移至较短的波长。该方法简单，荧光在 425/534nm 的激发/发射波长下最佳测量，在 50μM 至 50mM 尿酸浓度范围内线性增加，检测限为 0.14μM，低于文献中报道的其他方法。
