Department of Otolaryngology, Dalin Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Chiayi, Taiwan.
School of Medicine, Tzu Chi University, Hualien, Taiwan.
Nutr Neurosci. 2019 Dec;22(12):840-849. doi: 10.1080/1028415X.2018.1456040. Epub 2018 Mar 29.
This study evaluated the bioactive composition of tempeh products and examined the effects of tempeh on BV-2 microglial cell cytotoxicity, neurotrophic effects, and expression of inflammatory genes. Tempeh products included soybean fermented by , soybean fermented through cocultivation with and , and red bean fermented through cocultivation with and (RT-C). We analyzed the bioactive contents of tempeh extracts and evaluated the effects of tempeh water extract on lipopolysaccharide (LPS)-treated BV-2 cells. The results showed that RT-C water extract had the highest concentrations of γ-aminobutyric acid (GABA) and anthocyanin. The tempeh water extracts, especially RT-C, reduced the formation of LPS-induced reactive oxygen species, downregulated the levels of nitric oxide synthase and phospho-cyclic-AMP response element-binding protein, and upregulated the expression of brain-derived neurotrophic factor (BDNF). Our data demonstrate that RT-C has the highest concentrations of GABA and anthocyanin, more effectively reduces oxidative stress and inflammation, and increases the expression of BDNF in LPS-induced BV-2 cells.
本研究评估了豆豉产品的生物活性成分，并考察了豆豉对 BV-2 小胶质细胞细胞毒性、神经营养作用和炎症基因表达的影响。豆豉产品包括由发酵的大豆、与共培养发酵的大豆、与共培养发酵的红豆（RT-C）。我们分析了豆豉提取物的生物活性成分，并评估了豆豉水提取物对脂多糖（LPS）处理的 BV-2 细胞的影响。结果表明，RT-C 水提取物中γ-氨基丁酸（GABA）和花青素的浓度最高。豆豉水提取物，特别是 RT-C，可减少 LPS 诱导的活性氧的形成，下调一氧化氮合酶和磷酸环化-AMP 反应元件结合蛋白的水平，并上调脑源性神经营养因子（BDNF）的表达。我们的数据表明，RT-C 具有最高浓度的 GABA 和花青素，更有效地减轻氧化应激和炎症，并增加 LPS 诱导的 BV-2 细胞中 BDNF 的表达。
