Kiadehi Masoomeh Selseleh Hassan, Amoozegar Mohammad Ali, Asad Sedigheh, Siroosi Maryam
Extremophiles Laboratory, Department of Microbiology, Faculty of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, P.O. Box 14155-6455, Tehran, Iran E-mail:
Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran.
Water Sci Technol. 2018 Mar;77(5-6):1602-1611. doi: 10.2166/wst.2018.040.
Azo dyes are being extensively used in textile industries, so finding a proper solution to decolorize them is of high importance. In order to find azo dye decolorizing strains among haloarchaea, which are well known for their tolerance to harsh environmental conditions, fifteen haloarchaeal strains were screened. Halogeometricum sp. strain A and Haloferax sp. strain B with the highest decolorization ability (95% and 91% for Remazol black B; both about 60% for Acid blue 161, respectively) were selected for further studies. It was shown that both strains were able to grow and decolorize the dye in a medium containing up to 5 M NaCl, with optimum decolorization activity at 2.5-3.4 M, pH 7, and a wide temperature range between 30 to 45 °C. Moreover, both strains were able to tolerate and decolorize up to 1,000 mg l Remazol black B. Also, they were able to survive in 5,000 mg l of the dye after 20 days' incubation. Glucose and yeast extract were found to be the best carbon and nitrogen sources in the decolorization medium for both strains. This is the first report studying decolorization of azo dyes using halophilic archaea.
偶氮染料在纺织工业中被广泛使用，因此找到一种合适的使其脱色的解决方案至关重要。为了在以耐受恶劣环境条件而闻名的嗜盐古菌中寻找偶氮染料脱色菌株，筛选了15株嗜盐古菌菌株。选择了脱色能力最高的嗜盐几何菌属菌株A和嗜盐富盐菌属菌株B（雷马素黑B的脱色率分别为95%和91%；酸性蓝161的脱色率均约为60%）进行进一步研究。结果表明，这两种菌株都能够在含有高达5M氯化钠的培养基中生长并使染料脱色，在2.5 - 3.4M、pH 7以及30至45°C的宽温度范围内具有最佳脱色活性。此外，这两种菌株都能够耐受并使高达1000mg/L的雷马素黑B脱色。而且，在培养20天后，它们能够在5000mg/L的染料中存活。葡萄糖和酵母提取物被发现是这两种菌株脱色培养基中最佳的碳源和氮源。这是第一篇关于使用嗜盐古菌对偶氮染料进行脱色研究的报告。
