Garvey J S, Thomas D G, Linton H J
Department of Biology, Syracuse University, New York 13210.
Experientia Suppl. 1987;52:335-42. doi: 10.1007/978-3-0348-6784-9_31.
A heterogeneous, double-antibody, fluorometric enzyme-linked immunosorbent assay (ELISA) is described for the detection and quantitation of metallothionein(MT) The protocol uses the same immunological reagents as the radioimmunoassay(RIA) developed previously in this laboratory; fluorescence replaces radioactivity for detection of the reference antigen in the specific binding reaction. Present results indicate that the developed ELISA has approximately the same range of capability in detecting and quantitating MT as is characteristic of the RIA. The ELISA has the advantage that the time required to perform a typical assay is significantly less than that required for the RIA.
本文描述了一种用于检测和定量金属硫蛋白（MT）的异质性双抗体荧光酶联免疫吸附测定（ELISA）方法。该方法使用与本实验室先前开发的放射免疫测定（RIA）相同的免疫试剂；在特异性结合反应中，用荧光代替放射性来检测参考抗原。目前的结果表明，所开发的ELISA在检测和定量MT方面的能力范围与RIA的特征大致相同。ELISA的优点是进行一次典型检测所需的时间明显少于RIA所需的时间。
