生物材料中金属硫蛋白的测定：5种现行方法的实验室间比较

Metallothionein-determination in biological materials: interlaboratory comparison of 5 current methods.

作者信息

Dieter H H, Müller L, Abel J, Summer K H

机构信息

Institute of Toxicology, University of Düsseldorf, FRG.

出版信息

Experientia Suppl. 1987;52:351-8. doi: 10.1007/978-3-0348-6784-9_33.

DOI:10.1007/978-3-0348-6784-9_33

PMID:2959526

Abstract

Five current methods of metallothionein (MT)-estimation in biological materials (Hg/TCA-, Cd/heme-, SH-assay, G 75-method and RIA) were compared. Uniform calibration was ensured by measuring the nitrogen-content of the standard-apoprotein after "Kjeldahl-digestion". Known amounts of rabbit standard Cd-MT I were recovered from identical rat S9-samples. In an overall rating, RIA and Cd/heme-assay performed best, whereas the other methods either underestimated (SH-, G 75) or overestimated (Hg/TCA) the 100%-expectation.

摘要

比较了目前用于生物材料中金属硫蛋白（MT）测定的五种方法（汞/三氯乙酸法、镉/血红素法、巯基测定法、G75法和放射免疫分析法）。通过“凯氏定氮消化”后测量标准脱辅基蛋白的氮含量来确保统一校准。从相同的大鼠S9样品中回收已知量的兔标准镉-MT I。总体评价中，放射免疫分析法和镉/血红素测定法表现最佳，而其他方法要么低估了（巯基测定法、G75法），要么高估了（汞/三氯乙酸法）预期的100%。