大鼠金属硫蛋白多基因家族。

Rat metallothionein multigene family.

作者信息

Andersen R D, Taplitz S J, Birren B W, Bristol G, Herschman H R

机构信息

Laboratory of Biomedical and Environmental Sciences, University of California, Los Angeles 90024.

出版信息

Experientia Suppl. 1987;52:373-84. doi: 10.1007/978-3-0348-6784-9_35.

DOI:10.1007/978-3-0348-6784-9_35

PMID:2959527

Abstract

Southern blot analysis of rat genomic DNA reveals the presence of numerous sequences homologous to the rat MT-1 gene. We have isolated and characterized by sequence analysis the rat MT-1 gene and three related processed pseudogenes. We discuss mechanisms by which these pseudogenes were formed. The rat MT-1 and MT-2 structural genes are shown to be separated by 4 kb of intergenic DNA. By sequence analysis of the MT-2 structural gene, we compare features of the MT gene sequences which regulate promoter activity, and which specify transcription termination and polyadenylation. From an examination of homologous sequences in the MT promoters, we suggest that there may be both regulatory features in common with the MT gene isotypes, and regulatory mechanisms which may allow for differential expression of these genes. We discuss possible applications of recombinant DNA technology to investigating both the expression of MT genes and the functioning of MT proteins, in a tissue specific manner and during development.

摘要

对大鼠基因组DNA进行Southern印迹分析，结果显示存在许多与大鼠MT-1基因同源的序列。我们通过序列分析分离并鉴定了大鼠MT-1基因和三个相关的加工假基因。我们讨论了这些假基因形成的机制。大鼠MT-1和MT-2结构基因被证明被4 kb的基因间DNA隔开。通过对MT-2结构基因的序列分析，我们比较了调节启动子活性、指定转录终止和聚腺苷酸化的MT基因序列的特征。通过检查MT启动子中的同源序列，我们认为MT基因亚型可能存在共同的调节特征，以及可能允许这些基因差异表达的调节机制。我们讨论了重组DNA技术在以组织特异性方式和发育过程中研究MT基因表达和MT蛋白功能方面的可能应用。