Yamada K, Kato H, Kanda N, Fujii-Kuriyama Y, Utakoji T, Itoh R
Division of Geriatric Health Science, National Institute of Health and Nutrition, Tokyo, Japan.
Biochim Biophys Acta. 1994 Nov 22;1219(3):581-91. doi: 10.1016/0167-4781(94)90216-x.
The metallothionein (MT) I and II genes were isolated from Chinese hamster cells and sequenced. The MT-II gene is located about 6 kb upstream of the MT-I gene and their arrangement is similar to those of the mouse and rat MT genes. The sequence of the Chinese hamster MT-I gene is highly homologous to those of the mouse and rat, particularly in their promoter regions of MT-I. However, the promoter region of MT-II has less homology with those of the mouse and rat due t to insertions and deletions. The MT-I and MT-II genes were equally amplified 4-8-times in the Cd-resistant Chinese hamster cells, suggesting that both genes are included in the same amplification unit. Cytogenetic analysis of Cd-resistant cells by in situ hybridization showed that they are randomly integrated into multiple sites on the chromosomes.
从中国仓鼠细胞中分离出金属硫蛋白（MT）I和II基因并进行测序。MT-II基因位于MT-I基因上游约6 kb处，它们的排列方式与小鼠和大鼠的MT基因相似。中国仓鼠MT-I基因的序列与小鼠和大鼠的高度同源，特别是在MT-I的启动子区域。然而，由于插入和缺失，MT-II的启动子区域与小鼠和大鼠的同源性较低。在对镉具有抗性的中国仓鼠细胞中，MT-I和MT-II基因均被等量扩增4至8倍，这表明这两个基因包含在同一个扩增单元中。通过原位杂交对镉抗性细胞进行细胞遗传学分析表明，它们随机整合到染色体的多个位点上。
