Price-Haughey J, Gedamu L
Department of Biology, University of Calgary, Alberta, Canada.
Experientia Suppl. 1987;52:465-9. doi: 10.1007/978-3-0348-6784-9_46.
The regulation of heavy metal induced gene expression was investigated in two fish cell lines: the rainbow trout hepatoma (RTH) and Chinook salmon embryo (CHSE) cells. The induction of metallothionein (MT) synthesis occurred in RTH cells exposed to zinc, and to a lesser extent, following exposure to cadmium. The time courses of MT synthesis were different for the different metal inducers, suggesting that MT may be differentially regulated in these cells. CHSE cells, unlike RTH cells, did not synthesize MT in response to metal treatment. Treatment of the cells with 5-azacytidine prior to heavy metal exposure resulted in the induction of MT synthesis. Since these cells were embryonic in origin, these findings raised the possibility that MT gene expression may be developmentally regulated in fish. Analysis of the entire spectrum of cellular proteins has revealed the synthesis of an unknown, 14,000 dalton, metal-inducible protein (MIP) and various stress proteins following exposure of fish cells to heavy metals.
虹鳟肝癌（RTH）细胞和奇努克鲑鱼胚胎（CHSE）细胞。在暴露于锌的RTH细胞中，金属硫蛋白（MT）合成被诱导，在暴露于镉后诱导程度较小。不同金属诱导剂诱导MT合成的时间进程不同，这表明MT在这些细胞中可能受到不同的调节。与RTH细胞不同，CHSE细胞在金属处理后不合成MT。在重金属暴露前用5-氮杂胞苷处理细胞导致MT合成的诱导。由于这些细胞起源于胚胎，这些发现增加了MT基因表达可能在鱼类中受到发育调节的可能性。对细胞蛋白质全谱的分析表明，鱼类细胞暴露于重金属后会合成一种未知的、14000道尔顿的金属诱导蛋白（MIP）和各种应激蛋白。
