Dumpala Pradeep R, Lawrence Mark L, Karsi Attila
The Rogosin Institute-Xenia Division, 740 Birch Road, Xenia, Ohio, 45385, USA.
Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi, 39762, USA.
J Aquat Anim Health. 2018 Mar;30(1):50-56. doi: 10.1002/aah.10007.
Edwardsiella ictaluri is a facultative, intracellular, gram-negative bacterium that causes enteric septicemia of catfish (ESC). Edwardsiella ictaluri is known to be resistant to defense mechanisms present in catfish serum, which might aid in its use of a host's bloodstream to become septicemic. However, the precise mechanisms of the survival of E. ictaluri in host serum are not known. Analysis of the response of E. ictaluri to the host serum treatment at a proteomic level might aid in the elucidation of its adaptation mechanisms against defense mechanisms present in catfish serum. Thus, the objective of this study was to identify differentially regulated proteins of E. ictaluri upon exposure to naïve catfish serum. Two-dimensional difference gel electrophoresis (2D-DIGE) followed by in-gel trypsin digestion and MALDI-TOF/TOF analysis were used for identification of differentially expressed E. ictaluri proteins. A total of 19 differentially regulated proteins (7 up- and 12 downregulated) were identified. Among those were four putative immunogenic proteins, two chaperones and eight proteins involved in the translational process, two nucleic acid degradation and integration proteins, two intermediary metabolism proteins, and one iron-ion-binding protein. Further research focusing on the functions of these differentially expressed proteins may reveal their roles in host adaptation by E. ictaluri.
爱德华氏菌是一种兼性、细胞内、革兰氏阴性细菌，可引起鲶鱼的肠道败血症（ESC）。已知爱德华氏菌对鲶鱼血清中存在的防御机制具有抗性，这可能有助于其利用宿主的血液引发败血症。然而，爱德华氏菌在宿主血清中存活的确切机制尚不清楚。在蛋白质组水平上分析爱德华氏菌对宿主血清处理的反应，可能有助于阐明其针对鲶鱼血清中防御机制的适应机制。因此，本研究的目的是鉴定暴露于未免疫鲶鱼血清后爱德华氏菌中差异调节的蛋白质。采用二维差异凝胶电泳（2D-DIGE），随后进行胶内胰蛋白酶消化和MALDI-TOF/TOF分析，以鉴定爱德华氏菌中差异表达的蛋白质。共鉴定出19种差异调节蛋白（7种上调和12种下调）。其中包括四种假定的免疫原性蛋白、两种伴侣蛋白和八种参与翻译过程的蛋白、两种核酸降解和整合蛋白、两种中间代谢蛋白和一种铁离子结合蛋白。进一步针对这些差异表达蛋白功能的研究，可能会揭示它们在爱德华氏菌宿主适应中的作用。
