School of Software Engineering, Beijing University of Technology, Beijing, China.
Channing Division of Network Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, USA.
Genomics. 2019 May;111(3):500-507. doi: 10.1016/j.ygeno.2018.03.009. Epub 2018 Mar 27.
Alcohol (EtOH) dosage and exposure time can affect gene expression. However, whether there exists synergistic effect is unknown. Here, we analyzed the hDPSC gene microarray dataset GSE57255 downloaded from Gene Expression Omnibus and found that the interaction between EtOH dosage and exposure time on gene expression are statistically significant for two probes: 201917_s_at near gene SLC25A36 and 217649_at near gene ZFAND5. GeneMania showed that SLC25A36 and ZFAND5 were related to 20 genes, three of which had alcohol-related functions. WebGestalt revealed that the 22 genes were enriched in 10 KEGG pathways, four of which are related to alcoholic diseases. We explored the possible nonlinear interaction effect and got 172 gene probes with significant p-values. However, no significantly enriched pathways based on the 172 probes were detected. Our analyses indicated a possible molecular mechanism that could help explain why alcohol consumption has both deleterious and beneficial effects on human health.
酒精（EtOH）剂量和暴露时间会影响基因表达。然而，是否存在协同作用尚不清楚。在这里，我们分析了从基因表达综合数据库（Gene Expression Omnibus）下载的 hDPSC 基因微阵列数据集 GSE57255，发现对于两个探针，EtOH 剂量和暴露时间对基因表达的相互作用具有统计学意义：靠近基因 SLC25A36 的 201917_s_at 和靠近基因 ZFAND5 的 217649_at。GeneMania 显示 SLC25A36 和 ZFAND5 与 20 个基因相关，其中 3 个具有与酒精相关的功能。WebGestalt 表明，这 22 个基因富集在 10 个 KEGG 途径中，其中 4 个与酒精性疾病有关。我们探讨了可能的非线性相互作用效应，得到了 172 个具有显著 p 值的基因探针。然而，基于这 172 个探针，没有检测到显著富集的途径。我们的分析表明了一种可能的分子机制，可以帮助解释为什么饮酒对人类健康既有有害又有益的影响。
