Yang Zhiqiang, Liu Yujian, Zhou Xiaolei
Department of General Surgery, Tianjin Medical University General Hospital, China.
Department of General Surgery, Tianjin Medical University General Hospital, China.
Transpl Immunol. 2018 Aug;49:20-27. doi: 10.1016/j.trim.2018.03.004. Epub 2018 Mar 27.
RNA interference (RNAi) plays a potential role in organ transplantation. Small hairpin RNA (shRNA) is an artificial RNA molecule with a tight hairpin turn that can be used to silence the expression of a target gene. We constructed shRNA targeting on the cluster of differentiation 80 (CD80, B7-1) and the cluster of differentiation 86 (CD86, B7-2) and transfected it into dendritic cells (DCs). Fluorescence real-time PCR and flow cytometry confirmed the gene-silencing effect. Interleukin-2 (IL-2) mRNA expression level decreased in T cells that were cocultured with pB7-shRNA-transfected DCs. For in-vivo experiment, we built mice models of abdominal heterotopic heart transplantation and transfused the models with pB7-shRNA-transfected donor-derived DCs. The survival time of the transplanted heart increased; the grade of organ rejection decreased. IL-2 mRNA expression level decreased and it was positively correlated with the grade of organ rejection.
RNA干扰（RNAi）在器官移植中发挥着潜在作用。小发夹RNA（shRNA）是一种具有紧密发夹环的人工RNA分子，可用于沉默靶基因的表达。我们构建了靶向分化簇80（CD80，B7-1）和分化簇86（CD86，B7-2）的shRNA，并将其转染到树突状细胞（DCs）中。荧光实时PCR和流式细胞术证实了基因沉默效果。与pB7-shRNA转染的DCs共培养的T细胞中白细胞介素-2（IL-2）mRNA表达水平降低。对于体内实验，我们建立了腹部异位心脏移植小鼠模型，并用pB7-shRNA转染的供体来源DCs对模型进行输注。移植心脏的存活时间延长；器官排斥反应等级降低。IL-2 mRNA表达水平降低，且与器官排斥反应等级呈正相关。
