Tsai Chung-Lin, Chiu Ying-Ming, Ho Tin-Yun, Hsieh Chin-Tung, Shieh Dong-Chen, Lee Yi-Ju, Tsay Gregory J, Wu Yi-Ying
Division of Cardiovascular Surgery, Taichung Veterans General Hospital, Taichung, Taiwan, R.O.C.
Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan, R.O.C.
Anticancer Res. 2018 Apr;38(4):2057-2067. doi: 10.21873/anticanres.12445.
BACKGROUND/AIM: Gastric cancer is one of the most common malignant cancers with a poor prognosis and high mortality rate worldwide. Current treatment of gastric cancer includes surgery and chemotherapy as the main modalities, but the potentially severe side-effects of chemotherapy present a considerable challenge. Gallic acid is a trihydroxybenzoic acid found to exert an anticancer effect against a variety of cancer cells. The purpose of this study was to determine the anti-cancer activity of Galla chinensis and its main component gallic acid on human gastric adenocarcinoma cells.
MTT assay and cell death ELISA were used to determine the apoptotic effect of Gallic Chinensis and gallic acid on human gastric adenocarcinoma cells. To determine the pathway and relevant components by which gallic acid-induced apoptosis is mediated through, cells were transfected with siRNA (Fas, FasL, DR5, p53) using Lipofectamine 2000. Reults: Gallic Chinensis and gallic acid induced apoptosis of human gastric adenocarcinoma cells. Gallic acid induced up-regulation of Fas, FasL, and DR5 expression in AGS cells. Transfection of cells with Fas, FasL, or DR5 siRNA reduced gallic acid-induced cell death. In addition, p53 was shown to be involved in gallic acid-mediated Fas, FasL, and DR5 expression as well as cell apoptosis in AGS cells.
These results suggest that gallic acid has a potential role in the treatment of gastric cancer.
背景/目的：胃癌是全球最常见的恶性肿瘤之一，预后较差且死亡率高。目前胃癌的治疗主要包括手术和化疗，但化疗可能产生的严重副作用带来了相当大的挑战。没食子酸是一种三羟基苯甲酸，已发现其对多种癌细胞具有抗癌作用。本研究的目的是确定五倍子及其主要成分没食子酸对人胃腺癌细胞的抗癌活性。
采用MTT法和细胞死亡ELISA法测定五倍子和没食子酸对人胃腺癌细胞的凋亡作用。为了确定没食子酸诱导凋亡所介导的途径及相关成分，使用Lipofectamine 2000将siRNA（Fas、FasL、DR5、p53）转染至细胞。结果：五倍子和没食子酸诱导人胃腺癌细胞凋亡。没食子酸诱导AGS细胞中Fas、FasL和DR5表达上调。用Fas、FasL或DR5 siRNA转染细胞可减少没食子酸诱导的细胞死亡。此外，p53被证明参与没食子酸介导的AGS细胞中Fas、FasL和DR5表达以及细胞凋亡。
这些结果表明没食子酸在胃癌治疗中具有潜在作用。
