Knufman N M, van der Laarse A, Vliegen H W, Brinkman C J
Dept. Cardiology, University Hospital Leiden, The Netherlands.
Res Commun Chem Pathol Pharmacol. 1987 Jul;57(1):15-32.
Administration of a single high dose or multiple low doses of isoproterenol (ISO) to rats induces myocardial necrosis and cardiac hypertrophy. To assess the extent of necrosis we measured myocardial activities of creatine kinase (CK) and aspartate aminotransferase (AST) in the first days after ISO-treatment. After a single high dose and multiple low doses of ISO, necrosis was 10% and 25%, respectively. To quantify the degree of hypertrophy we measured heart weight and myocyte volume, and calculated cardiac CK, AST and DNA content up to 50 days after ISO-treatment. After a single high dose and multiple low doses of ISO, myocyte volume increased by 27% and 92%, respectively, while heart weight increased by 15% and 56%, respectively. As in this model hypertrophy is associated with focal necrosis, heart weight gain underestimates the actual increase of myocyte volume.
给大鼠单次注射高剂量或多次注射低剂量的异丙肾上腺素（ISO）会导致心肌坏死和心脏肥大。为了评估坏死程度，我们在ISO处理后的头几天测量了肌酸激酶（CK）和天冬氨酸转氨酶（AST）的心肌活性。单次注射高剂量和多次注射低剂量的ISO后，坏死率分别为10%和25%。为了量化肥大程度，我们测量了心脏重量和心肌细胞体积，并计算了ISO处理后长达50天的心脏CK、AST和DNA含量。单次注射高剂量和多次注射低剂量的ISO后，心肌细胞体积分别增加了27%和92%，而心脏重量分别增加了15%和56%。由于在该模型中肥大与局灶性坏死相关，心脏重量增加低估了心肌细胞体积的实际增加。
