Tong K Z, Wang W, Zhang X Y
Institute of Genetics, Academia Sinica, Beijing.
Sci Sin B. 1987 May;30(5):495-502.
Spontaneous streptomycin-dependent mutants (StrDA) were isolated from Escherichia coli C600. On C600 StrD, the lytic growth of phage lambda Nam and lambda cI857 was inhibited. After E. coli lysogenic strain 1.1485 (lambda cI857) mutated to StrDA, induction of lambda was decreased greatly. On StrDA of E. coli strains C600 and 1.1485 (lambda cI857), the plating efficiencies and burst sizes of phage T4 and T7 remained normal. Since StrDA is a mutation in the structural gene for ribosomal protein S12, the results obtained in the present study suggest that ribosomes of the StrDA mutants inhibit the lytic growth of lambda phage. The possibility that StrDA ribosomes inhibit the expression of lambda N gene is discussed based on the comparison of the genetic background of lambda cI857 and lambda Nam.
从大肠杆菌C600中分离出了自发的链霉素依赖性突变体（StrDA）。在C600 StrD上，噬菌体λNam和λcI857的裂解生长受到抑制。大肠杆菌溶原性菌株1.1485（λcI857）突变为StrDA后，λ的诱导显著降低。在大肠杆菌菌株C600和1.1485（λcI857）的StrDA上，噬菌体T4和T7的平板效率和爆发量保持正常。由于StrDA是核糖体蛋白S12结构基因中的一个突变，本研究获得的结果表明，StrDA突变体的核糖体抑制了λ噬菌体的裂解生长。基于λcI857和λNam遗传背景的比较，讨论了StrDA核糖体抑制λN基因表达的可能性。
