Kelly Peter
Axis Shield, Dundee, Scotland, UK.
Methods Mol Biol. 2018;1756:281-293. doi: 10.1007/978-1-4939-7734-5_24.
The technique of surface-enhanced laser desorption/ionization time-of-flight SELDI-TOF mass spectrometry could be used to establish serum or plasma proteomic profiles in esophageal adenocarcinoma. The protein profiles in patients may be used to predict survival and monitor response to chemotherapy in patients with esophageal adenocarcinoma. Here, a protocol for sample preparation from esophageal adenocarcinoma, analysis of proteomic profiles, and collection of protein fractions for identification of significant peaks is presented.
表面增强激光解吸/电离飞行时间质谱（SELDI-TOF-MS）技术可用于建立食管腺癌的血清或血浆蛋白质组图谱。患者的蛋白质图谱可用于预测食管腺癌患者的生存率并监测其对化疗的反应。本文介绍了一种从食管腺癌中制备样本、分析蛋白质组图谱以及收集蛋白质组分以鉴定显著峰的方案。
