使用一氧化氮选择性电极测量卵母细胞内一氧化氮浓度

Measurements of Intra-oocyte Nitric Oxide Concentration Using Nitric Oxide Selective Electrode.

作者信息

Khan Sana N, Jeelani Roohi, Abu-Soud Husam M

机构信息

Department of Obstetrics and Gynecology, C. S. Mott Center for Human Growth and Development, Wayne State University, Detroit, MI, USA.

Department of Biochemistry and Molecular Biology, Wayne State University, Detroit, MI, USA.

出版信息

Methods Mol Biol. 2018;1747:13-21. doi: 10.1007/978-1-4939-7695-9_2.

DOI:10.1007/978-1-4939-7695-9_2

PMID:29600447

Abstract

Precise information about the intracell nitric oxide (NO) concentration [NO] of a single cell are necessary in designing accurate experiments to further knowledge and develop treatment plans in certain disorders. The direct quantitative measurement of [NO] in situ in an intact cellular complex should be useful in tracking real-time and rapid changes at nanomolar levels. In this work, we describe the direct, real-time, and quantitative intracellular [NO] measurement utilizing an L-shaped amperometric integrated NO-selective electrode. This method not only provides an elegant and convenient approach to real-time the measurement of NO in physiological environments but also mimics the loss of NO caused by rapid NO diffusion combined with its reactivity in the biological milieu.

摘要

在设计精确实验以增进对某些疾病的了解并制定治疗方案时，单个细胞内一氧化氮（NO）浓度[NO]的精确信息是必要的。在完整细胞复合体中原位直接定量测量[NO]，对于追踪纳摩尔水平的实时快速变化应是有用的。在这项工作中，我们描述了利用L形安培法集成NO选择性电极进行直接、实时和定量的细胞内[NO]测量。这种方法不仅为在生理环境中实时测量NO提供了一种优雅且便捷的途径，还模拟了快速NO扩散及其在生物环境中的反应性所导致的NO损失。