Goodwin John M, Chrestensen Carol A, Moomaw Ellen W
Department of Chemistry and Biochemistry, Kennesaw State University, Kennesaw, GA, USA.
Methods Mol Biol. 2018;1747:35-47. doi: 10.1007/978-1-4939-7695-9_4.
Membrane inlet mass spectrometry (MIMS) is a reproducible and reliable method for the measurement of nitric oxide in aqueous solution with a lower limit of detection of 10 nM and a linear response to 50 μM. MIMS utilizes a semipermeable membrane to partition analytes based on physicochemical properties from the bulk sample into the mass spectrometer. Silastic tubing allows the introduction of small gaseous molecules including nitric oxide (NO) into the high vacuum of a mass spectrometer. We describe the measurement of NO generated chemically from nitrite and MAHMA NONOate as well as enzymatically by nitric oxide synthase (NOS).
膜进样质谱法（MIMS）是一种可重复且可靠的方法，用于测量水溶液中的一氧化氮，检测下限为10 nM，对50 μM呈线性响应。MIMS利用半透膜根据物理化学性质将分析物从大量样品中分离出来并引入质谱仪。硅橡胶管可将包括一氧化氮（NO）在内的小气态分子引入质谱仪的高真空环境中。我们描述了由亚硝酸盐和MAHMA NONOate化学产生的一氧化氮以及由一氧化氮合酶（NOS）酶促产生的一氧化氮的测量方法。
