Department of Radiology, Yueyang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Department of Radiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
J Cell Biochem. 2018 Jul;119(7):5805-5812. doi: 10.1002/jcb.26766. Epub 2018 Mar 30.
Giant cell tumor stromal cell (GCTSC) is the tumor cell of giant cell tumor of bone (GCTB). The biomarkers characterization of GCTSC is critical for the selection of GCTB targeting drugs. We believe the main functions of GCTSC in different part of tumor should be different for different environment. Then the biological behavior and molecular biomarkers of GCTSC should be different as well. Based on this idea, we focused on GCTSC which located in central tissue, peripheral tissue and took MMP-9 as the breakthrough point to carry out research. The results showed MMP-9 staining grade of GCTSC which located in central tissue was slight, whereas multinucleated giant cell staining grade was high. The peripheral tissue was consisted by almost GCTSC with high MMP-9 staining degree and mRNA expression. This study also provided clues and inspiration for reducing GCTB recurrence rate after intralesional curettage with MMP-9 targeted therapy which were aimed at the residual peripheral tissue.
破骨细胞样基质细胞（GCTSC）是骨巨细胞瘤（GCTB）的肿瘤细胞。GCTSC 生物标志物特征对于 GCTB 靶向药物的选择至关重要。我们认为，不同环境下肿瘤不同部位的 GCTSC 主要功能应该不同，其生物学行为和分子标志物也应该不同。基于这一想法，我们专注于位于中央组织、外周组织的 GCTSC，并以 MMP-9 为突破口开展研究。结果表明，位于中央组织的 GCTSC 的 MMP-9 染色程度较轻，而多核巨细胞的染色程度较高。外周组织主要由高 MMP-9 染色程度和 mRNA 表达的 GCTSC 组成。这项研究还为针对残留外周组织的 MMP-9 靶向治疗提供了减少肿瘤内刮除术后 GCTB 复发率的线索和启示。
