Lu Xiao-Feng, Dai Dong-Mei, Yu Rong-Min, Song Li-Yan, Zhu Jian-Hua, Fan Xiao-Na, Zi Jia-Chen
Biotechnological Institute of Chinese Materia Medica, Jinan University, Guangzhou 510632, China.
School of Biological and Chemical Engineering, Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, China.
Zhongguo Zhong Yao Za Zhi. 2018 Feb;43(3):537-543. doi: 10.19540/j.cnki.cjcmm.20180104.010.
Eight limonoids were isolated from 95% ethanol extracts of neem(Azadirachta indica) seeds by various chromatographic methods. By comparison of their spectroscopic data with those reported in the literatures, these limonoids were determined as salannin(1), 1-detigloyl-1-isobutylsalannin(2), salannol-3-acetate(3), salannol(4), spirosendan(5), 1-detigloyloxy-3-deacetylsalannin-1-en-3-one(6), nimbin(7) and 6-deacetylnimbin(8). Compounds 2 and 5 were firstly isolated from this genus and 5 represented the only example of its type. And 6 is a new natural product. 6 showed inhibitory activity against HeLa and HL-60 cells, with IC₅₀ of(21.61±4.37) and(27.33±5.74) μmol·L⁻¹, respectively. Both 7 and 8 mildly inhibited the growth of HeLa cells, with IC₅₀ of (33.15±5.24) and (38.56±6.41) μmol·L⁻¹, respectively.
通过多种色谱方法从印楝（Azadirachta indica）种子的95%乙醇提取物中分离得到了8种柠檬苦素。通过将它们的光谱数据与文献报道的数据进行比较，确定这些柠檬苦素为印楝素（1）、1 - 去tigloyl - 1 - 异丁基印楝素（2）、印楝醇 - 3 - 乙酸酯（3）、印楝醇（4）、螺旋苦楝素（5）、1 - 去tigloyloxy - 3 - 去乙酰印楝素 - 1 - 烯 - 3 - 酮（6）、尼姆比因（7）和6 - 去乙酰尼姆比因（8）。化合物2和5首次从该属中分离得到，且5是其类型中的唯一实例。6是一种新的天然产物。6对HeLa和HL - 60细胞显示出抑制活性，IC₅₀分别为（21.61±4.37）和（27.33±5.74）μmol·L⁻¹。7和8均对HeLa细胞的生长有轻度抑制作用，IC₅₀分别为（33.15±5.24）和（38.56±6.41）μmol·L⁻¹。
