Department of Chemistry , University of Tennessee Knoxville , 1420 Circle Drive , Knoxville , Tennessee 37996 , United States.
ACS Chem Neurosci. 2018 Jun 20;9(6):1380-1387. doi: 10.1021/acschemneuro.8b00020. Epub 2018 Apr 5.
The development of a sensor for the rapid and sensitive detection of neurotransmitters could provide a pathway for the diagnosis of neurological diseases, leading to the discovery of more effective treatment methods. We investigate the use of surface enhanced Raman spectroscopy (SERS) based sensors for the rapid detection of melatonin, serotonin, glutamate, dopamine, GABA, norepinephrine, and epinephrine. Previous studies have demonstrated SERS detection of neurotransmitters; however, there has been no comprehensive study on the effect of the metal used as the SERS substrate or the excitation wavelength used for detection. Here, we present the detection of 7 neurotransmitters using both silver and gold nanoparticles at excitation wavelengths of 532, 633, and 785 nm. Over the range of wavelengths investigated, the SERS enhancement on the silver and gold nanoparticles varies, with an average enhancement factor of 10-10. The maximum SERS enhancement occurs at an excitation wavelength of 785 nm for the gold nanoparticles and at 633 nm for the silver nanoparticles.
用于快速灵敏检测神经递质的传感器的开发可以为神经疾病的诊断提供途径，从而发现更有效的治疗方法。我们研究了基于表面增强拉曼光谱（SERS）的传感器用于快速检测褪黑素、血清素、谷氨酸、多巴胺、GABA、去甲肾上腺素和肾上腺素。以前的研究已经证明了 SERS 对神经递质的检测；然而，对于用作 SERS 基底的金属或用于检测的激发波长，还没有进行全面的研究。在这里，我们使用银和金纳米粒子在 532、633 和 785nm 的激发波长下检测了 7 种神经递质。在所研究的波长范围内，银和金纳米粒子的 SERS 增强程度不同，平均增强因子为 10-10。金纳米粒子的最大 SERS 增强发生在 785nm 的激发波长，银纳米粒子的最大 SERS 增强发生在 633nm 的激发波长。
