B-RCA 显示，在 ASCVD 高危 2 型糖尿病患者中，循环 miR-33a/b 与血清胆固醇相关。

B-RCA revealed circulating miR-33a/b associates with serum cholesterol in type 2 diabetes patients at high risk of ASCVD.

机构信息

Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Donghu Road 169#, Wuhan 430071, China.

College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan 430072, Hubei, China.

出版信息

Diabetes Res Clin Pract. 2018 Jun;140:191-199. doi: 10.1016/j.diabres.2018.03.024. Epub 2018 Mar 27.

DOI:10.1016/j.diabres.2018.03.024

PMID:29601916

Abstract

AIMS

Type 2 diabetes (T2D) is a complex metabolic disease with high incidence throughout the world. Dyslipidemia is the leading cause of atherosclerotic cardiovascular diseases (ASCVD) in T2D patients. hsa-miR-33 (miR-33) serves as a regulator in lipid metabolism. We hypothesized that blood miR-33 associates with serum lipids in T2D patients at high risk of ASCVD events.

METHODS

We developed a branched rolling circle amplification (B-RCA) method and assessed its sensitivity and specificity with miR-33a/b standards by traditional TaqMan assay. Circulating miR-33a/b level was then determined with B-RCA in 30 T2D patients at high risk for developing ASCVD and 33 healthy controls. Pearson correlation coefficient was used to evaluate the correlation between circulating miR-33a/b and serum cholesterol.

RESULTS

Compared with TaqMan assay, B-RCA method showed a similar specificity and a 100-fold higher sensitivity for miR-33a detection. Circulating miR-33a/b level is positively correlated with serum total cholesterol (TC) (r = 0.364, p = 0.048) and low-density lipoprotein cholesterol (LDL-C) (r = 0.383, p = 0.037) in T2D patients at high risk for developing ASCVD.

CONCLUSIONS

Our B-RCA method provided an alternative strategy with specificity and high sensitivity for circulating miRNAs detection, and the results demonstrated that miR-33a/b might play an important role in cholesterol regulation.

摘要

目的

2 型糖尿病（T2D）是一种复杂的代谢性疾病，在全球范围内发病率很高。血脂异常是 T2D 患者发生动脉粥样硬化性心血管疾病（ASCVD）的主要原因。hsa-miR-33（miR-33）作为脂质代谢的调节剂。我们假设 T2D 患者发生 ASCVD 事件风险较高时，血液 miR-33 与血清脂质有关。

方法

我们开发了一种分支滚环扩增（B-RCA）方法，并通过传统的 TaqMan 测定法用 miR-33a/b 标准品评估其灵敏度和特异性。然后，我们用 B-RCA 法测定了 30 例 ASCVD 高危 T2D 患者和 33 例健康对照者的循环 miR-33a/b 水平。Pearson 相关系数用于评估循环 miR-33a/b 与血清胆固醇之间的相关性。

结果

与 TaqMan 测定法相比，B-RCA 法检测 miR-33a 的特异性相似，但灵敏度高 100 倍。在 ASCVD 高危 T2D 患者中，循环 miR-33a/b 水平与血清总胆固醇（TC）（r=0.364，p=0.048）和低密度脂蛋白胆固醇（LDL-C）（r=0.383，p=0.037）呈正相关。