Facilitation of yeast-lethal membrane protein production by detoxifying with GFP tagging.

作者信息

Oshikane Hiroyuki, Watabe Masahiko, Nakaki Toshio

机构信息

Department of Pharmacology, Teikyo University School of Medicine, Kaga 2-11-1, Itabashi-ku, Tokyo 173-8605, Japan.

Department of Pharmacology, Teikyo University School of Medicine, Kaga 2-11-1, Itabashi-ku, Tokyo 173-8605, Japan; General Medical Education and Research Center (G-MEC), Teikyo University, Kaga 2-11-1, Itabashi-ku, Tokyo 173-8605, Japan.

出版信息

Protein Expr Purif. 2018 Aug;148:40-45. doi: 10.1016/j.pep.2018.03.011. Epub 2018 Mar 27.

DOI:10.1016/j.pep.2018.03.011

PMID:29601966

Abstract

Recombinant techniques for target protein production have been rapidly established and widely utilised in today's biological research. Nevertheless, methods for membrane protein production have yet to be developed, since membrane proteins generally tend to be expressed at low levels, easily aggregated, and/or even toxic to their host cells. Here we report that a GFP-tagging technique can be applied for the stable production of membrane proteins that are toxic to their host cells when overexpressed, paving the way for future advances in membrane protein biochemistry and drug development.

摘要