肿瘤游离环状 DNA（ecDNA）作为血液液体活检的一个组成部分的存在。
The presence of tumor extrachomosomal circular DNA (ecDNA) as a component of liquid biopsy in blood.
机构信息
Chronic Diseases Research Center, Endocrinology and Metabolism Population Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
出版信息
Med Hypotheses. 2018 May;114:5-7. doi: 10.1016/j.mehy.2018.02.018. Epub 2018 Feb 26.
In molecular biology covalently closed circular DNAs are able to passthrough double layer of eukaryotic cellular membrane. Very recently the presence of circular extra chromosomal DNA (ecDNA) has been shown which are different in seventeen different types of cancers. In fact, ecDNA are the tricky way of oncogenes to increase their copy number. We hypothesis the presence of ecDNA in the blood of cancer patients as a subpopulation of liquid biopsy. On the occasion of their presence in blood they will be very beneficial to cover the small amount of cell frees DNA (cfDNA). Isolation and characterization of ecDNA will be possible by a sensitive method entitled Circle-Seq. The origin of tumor more than its prognosis and diagnosis will be possible in the easiest way by using ecDNA.
在分子生物学中，共价闭合的环状 DNA 能够穿透真核细胞的双层膜。最近，已经发现了存在于 17 种不同类型癌症中的环状染色体外 DNA（ecDNA）。事实上，ecDNA 是致癌基因增加其拷贝数的一种狡猾方式。我们假设 ecDNA 作为液体活检的一个亚群存在于癌症患者的血液中。在它们存在于血液中的情况下，它们将非常有助于覆盖少量的游离细胞 DNA（cfDNA）。通过一种名为 Circle-Seq 的敏感方法，ecDNA 的分离和特征分析将成为可能。通过使用 ecDNA，可以以最简单的方式了解肿瘤的起源，而不仅仅是其预后和诊断。
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