Department of Biochemistry, University of Alberta, Edmonton, Alberta T6G 2P5, Canada.
J Biol Chem. 2018 Mar 30;293(13):4664-4665. doi: 10.1074/jbc.H118.002210.
Decades of work have contributed to our in-depth mechanistic understanding of soluble proteases, but much less is known about the catalytic mechanism of intramembrane proteolysis due to inherent difficulties in both preparing and analyzing integral membrane enzymes and transmembrane substrates. New work from Naing tackles this challenge by examining the catalytic parameters of an aspartyl intramembrane protease homologous to the enzyme that cleaves amyloid precursor protein, finding that both chemistry and register contribute to specificity in substrate cleavage.
几十年来的研究工作使我们深入了解了可溶性蛋白酶的作用机制，但由于制备和分析完整膜酶和跨膜底物固有困难，对于跨膜蛋白水解的催化机制知之甚少。Naing 的新研究通过研究一种天冬氨酰基跨膜蛋白酶的催化参数来应对这一挑战，这种蛋白酶与切割淀粉样前体蛋白的酶具有同源性，研究发现化学和登记都有助于底物切割的特异性。
