Center for Molecular Biology, Institute of Research and Development, Duy Tan University, 03 Quang Trung Street, Da Nang City, Vietnam.
Department of Life Science and Biochemical Engineering, Sun Moon University, 70 Sunmoon-ro 221, Tangjeong-myeon, Asan-si, Chungnam, 31460, Republic of Korea.
Appl Microbiol Biotechnol. 2018 May;102(10):4355-4370. doi: 10.1007/s00253-018-8957-x. Epub 2018 Mar 30.
Streptomyces peucetius ATCC 27952 produces two major anthracyclines, doxorubicin (DXR) and daunorubicin (DNR), which are potent chemotherapeutic agents for the treatment of several cancers. In order to gain detailed insight on genetics and biochemistry of the strain, the complete genome was determined and analyzed. The result showed that its complete sequence contains 7187 protein coding genes in a total of 8,023,114 bp, whereas 87% of the genome contributed to the protein coding region. The genomic sequence included 18 rRNA, 66 tRNAs, and 3 non-coding RNAs. In silico studies predicted ~ 68 biosynthetic gene clusters (BCGs) encoding diverse classes of secondary metabolites, including non-ribosomal polyketide synthase (NRPS), polyketide synthase (PKS I, II, and III), terpenes, and others. Detailed analysis of the genome sequence revealed versatile biocatalytic enzymes such as cytochrome P450 (CYP), electron transfer systems (ETS) genes, methyltransferase (MT), glycosyltransferase (GT). In addition, numerous functional genes (transporter gene, SOD, etc.) and regulatory genes (afsR-sp, metK-sp, etc.) involved in the regulation of secondary metabolites were found. This minireview summarizes the genome-based genome mining (GM) of diverse BCGs and genome exploration (GE) of versatile biocatalytic enzymes, and other enzymes involved in maintenance and regulation of metabolism of S. peucetius. The detailed analysis of genome sequence provides critically important knowledge useful in the bioengineering of the strain or harboring catalytically efficient enzymes for biotechnological applications.
吸水链霉菌 ATCC 27952 产生两种主要的蒽环类抗生素，阿霉素（DXR）和柔红霉素（DNR），它们是治疗多种癌症的有效化疗药物。为了深入了解该菌株的遗传学和生物化学，我们确定并分析了其完整基因组。结果表明，其完整序列包含 7187 个编码蛋白的基因，共计 8023114bp，而基因组的 87%贡献给了蛋白编码区。基因组序列包括 18 个 rRNA、66 个 tRNA 和 3 个非编码 RNA。计算机研究预测了约 68 个生物合成基因簇（BCG），它们编码多种类别的次级代谢产物，包括非核糖体聚酮合酶（NRPS）、聚酮合酶（PKS I、II 和 III）、萜类化合物和其他物质。对基因组序列的详细分析揭示了多种生物催化酶，如细胞色素 P450（CYP）、电子传递系统（ETS）基因、甲基转移酶（MT）和糖基转移酶（GT）。此外，还发现了许多参与次级代谢物调节的功能基因（转运基因、SOD 等）和调节基因（afsR-sp、metK-sp 等）。本文综述了基于基因组的吸水链霉菌不同 BCG 的基因挖掘（GM）和多功能生物催化酶的基因组探索（GE），以及其他参与代谢维持和调节的酶。基因组序列的详细分析为该菌株的生物工程或为生物技术应用提供催化效率高的酶提供了至关重要的知识。
