胎儿发育过程中的非人灵长类动物肾脏转录组
The non-human primate kidney transcriptome in fetal development.
作者信息
Spradling-Reeves Kimberly D, Glenn Jeremy P, Lange Kenneth J, Kuhn Natalia, Coalson Jacqueline J, Nijland Mark J, Li Cun, Nathanielsz Peter W, Cox Laura A
机构信息
Department of Internal Medicine, Wake Forest University Health Sciences, Winston-Salem, NC, USA.
Department of Genetics, Texas Biomedical Research Institute, San Antonio, TX, USA.
出版信息
J Med Primatol. 2018 Jun;47(3):157-171. doi: 10.1111/jmp.12340. Epub 2018 Mar 30.
BACKGROUND
Little is known about the repertoire of non-human primate kidney genes expressed throughout development. The present work establishes an understanding of the primate renal transcriptome during fetal development in the context of renal maturation.
METHODS
The baboon kidney transcriptome was characterized at 60-day gestation (DG), 90 DG, 125 DG, 140 DG, 160 DG and adulthood (6-12 years) using gene arrays and validated by QRT-PCR. Pathway and cluster analyses were used to characterize gene expression in the context of biological pathways.
RESULTS
Pathway analysis indicated activation of pathways not previously reported as relevant to kidney development. Cluster analysis also revealed gene splice variants with discordant expression profiles during development.
CONCLUSIONS
This study provides the first detailed genetic analysis of the developing primate kidney, and our findings of discordant expression of gene splice variants suggest that gene arrays likely provide a simplified view and demonstrate the need to study the fetal renal proteome.
背景
关于非人灵长类动物肾脏基因在整个发育过程中的表达谱，我们了解得很少。目前的工作是在肾脏成熟的背景下，建立对灵长类动物胎儿发育过程中肾脏转录组的认识。
方法
使用基因芯片对狒狒在妊娠60天（DG）、90 DG、125 DG、140 DG、160 DG和成年期（6 - 12岁）的肾脏转录组进行表征，并通过定量逆转录聚合酶链反应（QRT-PCR）进行验证。通路和聚类分析用于在生物学通路的背景下表征基因表达。
结果
通路分析表明激活了以前未报道与肾脏发育相关的通路。聚类分析还揭示了在发育过程中具有不一致表达谱的基因剪接变体。
结论
本研究首次对发育中的灵长类动物肾脏进行了详细的基因分析，我们关于基因剪接变体不一致表达的发现表明，基因芯片可能提供了一个简化的视图，并证明有必要研究胎儿肾脏蛋白质组。
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