微小RNA-7、微小RNA-9和微小RNA-375有助于艾塞那肽-4对高脂饮食喂养小鼠胰腺β细胞的作用。
MiR-7, miR-9 and miR-375 contribute to effect of Exendin-4 on pancreatic β-cells in high-fat-diet-fed mice.
作者信息
Guo Chang, Sun Yi-Qiong, Li Qiang, Zhang Jin-Chao
机构信息
Department of Endocrinology and Metabolism, Second Affiliated Hospital of Harbin Medical University, Harbin, China.
出版信息
Clin Invest Med. 2018 Mar 27;41(1):E16-E24. doi: 10.25011/cim.v41i1.29459.
PURPOSE
The purpose of this study was to test whether glucagon-like peptide-1 (GLP-1) receptor activation preserved pancreatic β-cells via the regulation of microRNAs and target genes in high-fat-diet-fed mice.
METHODS
C57BL/6 male mice were simultaneously treated with high-fat-diet (HFD) and GLP-1 analogue, Exendin-4 (Ex-4) (3 μg/kg/day or 30 μg/kg/day), i.p. or vehicle, for consecutive 13 weeks. Fasting blood glucose, postprandial blood glucose, ΔI30/ΔG30, HOMA-IR and HOMA-% β were measured in each group. Pancreatic β-cell mass was assessed by immunohistochemistry. The expression of miRNAs and related downstream genes were investigated using quantitative real-time PCR.
RESULTS
Thirteen weeks of Ex-4 treatment significantly reduced body weight and food intake in HFD-fed mice (P.
目的
本研究旨在测试胰高血糖素样肽-1（GLP-1）受体激活是否通过调节高脂饮食喂养小鼠中的微小RNA和靶基因来保护胰腺β细胞。
方法
C57BL/6雄性小鼠同时接受高脂饮食（HFD）和GLP-1类似物艾塞那肽-4（Ex-4）（3μg/kg/天或30μg/kg/天）腹腔注射或溶剂对照，连续13周。测量每组的空腹血糖、餐后血糖、ΔI30/ΔG30、HOMA-IR和HOMA-%β。通过免疫组织化学评估胰腺β细胞质量。使用定量实时PCR研究微小RNA和相关下游基因的表达。
结果
Ex-4治疗13周显著降低了高脂饮食喂养小鼠的体重和食物摄入量（P.
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