Chemistry Department, Universidade Federal de Lavras (UFLA), Lavras, Brazil.
Food Science Department, Universidade Federal de Lavras (UFLA), Lavras, Brazil.
Chem Biol Drug Des. 2018 Jul;92(1):1333-1343. doi: 10.1111/cbdd.13198. Epub 2018 Apr 23.
In this study, different phenolic extracts were obtained from the jaboticaba skin meal (JSM), whose phenolic compounds were characterized and their antibacterial activities were assessed. Moreover, the activity of lyophilized ethanolic extract of jaboticaba skin (EEJS) on wound healing was analyzed in rats. The JSM phenolic extracts were obtained in four ways: aqueous, methanolic, ethanolic, and acetone extracts. The phenolic compounds were characterized in these extracts by high-performance liquid chromatography, and their antibacterial activities were evaluated. The in vivo experiment was divided into four groups and received the following treatments: G1-silver sulfadiazine (positive control); G2-EEJS at 10%; G3-EEJS at 5%, and G4-EEJS at 2.5%. The aqueous extract did not inhibit the growing of any bacterium. The ethanolic, acetone, and methanolic extracts inhibited the growing of all bacteria tested at the concentrations of 1.25%, 2.50%, and 5.00%, respectively. The ethanolic extract was the one that showed the highest bacterial inhibition potential and the highest contents of phenolic compounds, especially of catechin, epicatechin gallate, and epicatechin. The G3 and G4 treatments presented faster wound healing compared to the G1 one, as it promoted a less intense inflammatory reaction and full closure of the wounds at an accelerated rate.
在这项研究中，从嘉宝果果皮粉（JSM）中获得了不同的酚类提取物，对其酚类化合物进行了表征，并评估了它们的抗菌活性。此外，还分析了冻干嘉宝果果皮乙醇提取物（EEJS）在大鼠伤口愈合中的活性。通过四种方法获得了 JSM 酚类提取物：水提物、甲醇提物、乙醇提物和丙酮提物。通过高效液相色谱法对这些提取物中的酚类化合物进行了表征，并评估了它们的抗菌活性。体内实验分为四组，分别接受以下处理：G1-磺胺嘧啶银（阳性对照）；G2-EEJS 浓度为 10%；G3-EEJS 浓度为 5%，G4-EEJS 浓度为 2.5%。水提物不能抑制任何细菌的生长。乙醇、丙酮和甲醇提取物在 1.25%、2.50%和 5.00%的浓度下分别抑制了所有测试细菌的生长。乙醇提取物具有最高的抑菌潜力和最高的酚类化合物含量，特别是儿茶素、没食子酸表儿茶素和表儿茶素。与 G1 相比，G3 和 G4 处理的伤口愈合更快，因为它促进了炎症反应减弱，伤口更快完全愈合。
