Luther A, Le Thi X, Schäfer J, Schulze M, Waberski D
Unit for Reproductive Medicine of Clinics/Clinic for Pigs and Small Ruminants, University of Veterinary Medicine Hannover, Hannover, Germany.
Institute for the Reproduction of Farm Animals Schönow Inc., Bernau, Germany.
Reprod Domest Anim. 2018 Aug;53(4):1016-1019. doi: 10.1111/rda.13186. Epub 2018 Mar 31.
Recent reports indicate that stimulation of liquid-stored boar semen with red LED-based light improves sperm quality and reproductive performance in sow herds. So far, in vitro data after LED stimulation of whole semen doses are lacking. In this study, the effect of LED light exposure on the in vitro quality of boar spermatozoa after storage and thermic incubation was examined. Boar semen doses were stored at 17°C (n = 10) or 5°C (n = 6) in Beltsville Thawing Solution extender and then exposed to red LED light using a commercial photo chamber. During a subsequent long-term incubation at 38°C, neither sperm kinematic parameters nor mitochondria function or membrane integrity differed between control and treated samples (p > .05). It is concluded that stimulation of semen doses in the LED-photo chamber does not improve quality of thermically stressed boar sperm in vitro. Other than the sperm traits tested here might be involved in the previously reported improvement of in vivo fertility.
近期报告表明，用基于红色发光二极管（LED）的光刺激液态保存的公猪精液可提高母猪群的精子质量和繁殖性能。到目前为止，缺乏关于LED刺激全剂量精液后的体外数据。在本研究中，检测了LED光照对储存和热孵育后公猪精子体外质量的影响。公猪精液剂量在Beltsville解冻液稀释剂中于17°C（n = 10）或5°C（n = 6）下储存，然后使用商用光照箱暴露于红色LED光下。在随后38°C的长期孵育过程中，对照样本和处理样本之间的精子运动参数、线粒体功能或膜完整性均无差异（p>0.05）。得出的结论是，在LED光照箱中刺激精液剂量并不能改善体外热应激公猪精子的质量。除了此处测试的精子特征外，先前报道的体内生育力改善可能还涉及其他因素。
